Experiment set5IT015 for Klebsiella michiganensis M5al

L-Glutamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Glutamine (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Mar-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1765 B2

Specific Phenotypes

For 16 genes in this experiment

For carbon source L-Glutamine in Klebsiella michiganensis M5al

For carbon source L-Glutamine across organisms

SEED Subsystems

Subsystem	#Specific
D-ribose utilization	2
Acetyl-CoA fermentation to Butyrate	1
Anaerobic respiratory reductases	1
Butanol Biosynthesis	1
DNA-binding regulatory proteins, strays	1
Deoxyribose and Deoxynucleoside Catabolism	1
Glycolate, glyoxylate interconversions	1
Histidine Degradation	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Isoleucine degradation	1
Photorespiration (oxidative C2 cycle)	1
Thioredoxin-disulfide reductase	1
Trehalose Uptake and Utilization	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Glutathione metabolism
• Glyoxylate and dicarboxylate metabolism
• Butanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ribose phosphorylation	2	2	1
glycolate and glyoxylate degradation II	2	2	1
2-deoxy-D-ribose degradation I	3	3	1
glycolate and glyoxylate degradation III	3	1	1
glycolate and glyoxylate degradation I	4	4	1
pyruvate fermentation to butanol II (engineered)	6	4	1
superpathway of glycol metabolism and degradation	7	7	1
fatty acid β-oxidation I (generic)	7	6	1
photorespiration I	9	5	1
photorespiration III	9	5	1
L-lysine degradation V	9	2	1
photorespiration II	10	6	1
pyruvate fermentation to hexanol (engineered)	11	8	1
oleate β-oxidation	35	32	3
1-butanol autotrophic biosynthesis (engineered)	27	19	1
superpathway of L-lysine degradation	43	12	1