Experiment set5IT015 for Bacteroides thetaiotaomicron VPI-5482

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D-Serine nitrogen source

Group: nitrogen source
Media: Varel_Bryant_medium_noNitrogen + D-Serine (20 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 12/19/17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)
Growth plate: 1942 D3

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source D-Serine in Bacteroides thetaiotaomicron VPI-5482

For nitrogen source D-Serine across organisms

SEED Subsystems

Subsystem #Specific
Heat shock dnaK gene cluster extended 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UTP and CTP dephosphorylation I 7 5 2
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 5 1
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 6 1
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 8 1
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 7 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 10 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 16 1