Experiment set5IT015 for Bifidobacterium breve UCC2003

Recovery broth with Trimethoprim 0.01 mM

Group: stress
Media: Recovery broth + Trimethoprim (0.01 mM), pH=6.8
Culturing: Bifido_ML2, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Anthony Shiver on 2/15/2020

Specific Phenotypes

For 3 genes in this experiment

For stress Trimethoprim in Bifidobacterium breve UCC2003

For stress Trimethoprim across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	1
One-carbon metabolism by tetrahydropterines	1
Serine-glyoxylate cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• One carbon pool by folate
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
folate transformations I	13	4	1