Experiment set5IT014 for Pseudomonas fluorescens FW300-N2E3

D-Glucosamine Hydrochloride carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Glucosamine Hydrochloride (20 mM), pH=7
Culturing: pseudo3_N2E3_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/17/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 13 genes in this experiment

For carbon source D-Glucosamine Hydrochloride in Pseudomonas fluorescens FW300-N2E3

For carbon source D-Glucosamine Hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	3
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis	2
D-gluconate and ketogluconates metabolism	1
Sialic Acid Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Aminosugars metabolism
• Pentose phosphate pathway
• Galactose metabolism
• Glycine, serine and threonine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-serine degradation	3	3	3
N-acetylglucosamine degradation I	2	2	2
L-cysteine degradation II	3	3	2
L-serine degradation	3	3	2
N-acetylglucosamine degradation II	3	2	2
L-tryptophan degradation II (via pyruvate)	3	2	2
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
L-methionine biosynthesis II	6	5	2
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	6	2	2
glycine betaine degradation III	7	7	2
glycine betaine degradation I	8	6	2
L-mimosine degradation	8	4	2
D-galactosamine and N-acetyl-D-galactosamine degradation	4	2	1
glutathione-mediated detoxification I	8	3	2
chitin derivatives degradation	8	2	2
N-acetyl-D-galactosamine degradation	5	2	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	5	1
chitin degradation I (archaea)	7	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
superpathway of N-acetylneuraminate degradation	22	15	2
purine nucleobases degradation II (anaerobic)	24	16	2
peptidoglycan recycling I	14	11	1