Experiment set5IT010 for Bacteroides thetaiotaomicron VPI-5482

L-aspartic acid nitrogen source

Group: nitrogen source
Media: Varel_Bryant_medium_noNitrogen + L-Aspartic Acid (6 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 12/19/17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution minus Nitrogen (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 1.75 mM Sodium sulfate)
Growth plate: 1942 A4

Specific Phenotypes

For 17 genes in this experiment

For nitrogen source L-Aspartic Acid in Bacteroides thetaiotaomicron VPI-5482

For nitrogen source L-Aspartic Acid across organisms

SEED Subsystems

Subsystem	#Specific
Fermentations: Mixed acid	2
Butanol Biosynthesis	1
DNA repair, bacterial MutL-MutS system	1
Folate Biosynthesis	1
One-carbon metabolism by tetrahydropterines	1
Serine-glyoxylate cycle	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism
• Propanoate metabolism
• Glycolysis / Gluconeogenesis
• Glyoxylate and dicarboxylate metabolism
• Butanoate metabolism
• One carbon pool by folate
• Reductive carboxylate cycle (CO2 fixation)

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetate and ATP formation from acetyl-CoA III	1	1	1
acetate conversion to acetyl-CoA	1	1	1
formate assimilation into 5,10-methylenetetrahydrofolate	3	3	2
reductive monocarboxylic acid cycle	2	2	1
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate	2	2	1
superpathway of acetate utilization and formation	3	3	1
pyruvate fermentation to acetate IV	3	3	1
reductive glycine pathway of autotrophic CO2 fixation	9	6	3
pyruvate fermentation to ethanol I	3	2	1
ethanol degradation II	3	2	1
L-isoleucine biosynthesis V	3	2	1
ethanol degradation IV	3	2	1
ethanol degradation III	3	1	1
formaldehyde oxidation VII (THF pathway)	4	4	1
chitin deacetylation	4	1	1
folate polyglutamylation	5	5	1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation	5	3	1
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)	10	3	2
folate transformations II (plants)	11	8	2
L-histidine degradation III	6	6	1
L-threonine degradation I	6	5	1
superpathway of bitter acids biosynthesis	18	3	3
colupulone and cohumulone biosynthesis	6	1	1
lupulone and humulone biosynthesis	6	1	1
adlupulone and adhumulone biosynthesis	6	1	1
folate transformations I	13	8	2
Arg/N-end rule pathway (eukaryotic)	14	6	2
folate transformations III (E. coli)	9	7	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	7	1
cis-geranyl-CoA degradation	9	1	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	10	1
purine nucleobases degradation I (anaerobic)	15	5	1
mixed acid fermentation	16	12	1
superpathway of L-threonine metabolism	18	13	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	14	1
superpathway of N-acetylneuraminate degradation	22	16	1
purine nucleobases degradation II (anaerobic)	24	12	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	2