Experiment set5IT009 for Pseudomonas fluorescens FW300-N1B4

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Cytosine nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf1N1B4_1307 -3.9 -2.7 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
Pf1N1B4_1408 -3.5 -5.1 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf1N1B4_1624 -3.5 -7.9 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf1N1B4_1610 -3.4 -6.4 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf1N1B4_2547 -3.1 -3.9 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf1N1B4_2880 -3.0 -12.9 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf1N1B4_2549 -3.0 -9.8 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf1N1B4_1534 -3.0 -18.2 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_1434 -3.0 -9.9 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf1N1B4_1831 -2.9 -11.4 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf1N1B4_2303 -2.9 -14.5 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf1N1B4_6 -2.9 -4.4 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf1N1B4_15 -2.8 -3.1 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf1N1B4_2338 -2.8 -9.0 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf1N1B4_1627 -2.8 -5.4 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf1N1B4_1067 -2.7 -4.8 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf1N1B4_2112 -2.6 -15.6 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf1N1B4_742 -2.6 -7.9 Argininosuccinate synthase (EC 6.3.4.5) compare
Pf1N1B4_1533 -2.6 -7.3 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf1N1B4_1626 -2.6 -7.2 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf1N1B4_2879 -2.6 -7.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf1N1B4_2545 -2.6 -12.6 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf1N1B4_2384 -2.5 -10.9 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf1N1B4_350 -2.5 -6.8 Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51) compare
Pf1N1B4_2548 -2.5 -6.1 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf1N1B4_5665 -2.4 -5.6 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf1N1B4_1830 -2.4 -8.6 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_2491 -2.4 -5.7 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf1N1B4_58 -2.3 -7.1 Transaldolase (EC 2.2.1.2) compare
Pf1N1B4_12 -2.3 -7.3 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf1N1B4_3833 -2.3 -9.5 Major porin and structural outer membrane porin OprF compare
Pf1N1B4_3081 -2.3 -18.1 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf1N1B4_1611 -2.3 -4.8 Nitrogen regulation protein NR(I) compare
Pf1N1B4_1625 -2.2 -4.5 FIG00956267: hypothetical protein compare
Pf1N1B4_2878 -2.2 -7.8 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_2272 -2.2 -4.3 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf1N1B4_847 -2.2 -8.7 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf1N1B4_2337 -2.2 -5.9 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf1N1B4_1315 -2.2 -7.9 IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) compare
Pf1N1B4_1565 -2.1 -4.8 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf1N1B4_2377 -2.1 -7.3 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf1N1B4_2858 -2.1 -10.5 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf1N1B4_2282 -2.1 -8.9 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf1N1B4_2011 -2.0 -9.9 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf1N1B4_2487 -2.0 -7.4 Biotin synthase (EC 2.8.1.6) compare
Pf1N1B4_3506 -2.0 -7.1 Cysteine synthase B (EC 2.5.1.47) compare
Pf1N1B4_2465 -2.0 -3.6 Two-component response regulator CreB compare
Pf1N1B4_2490 -2.0 -6.7 Biotin synthesis protein BioC compare
Pf1N1B4_3518 -1.9 -3.4 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94) compare
Pf1N1B4_6041 -1.9 -3.5 Carbon storage regulator compare
Pf1N1B4_2010 -1.9 -6.2 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf1N1B4_2488 -1.9 -7.8 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf1N1B4_2489 -1.8 -6.0 Biotin synthesis protein BioH compare
Pf1N1B4_3812 -1.8 -7.9 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10) compare
Pf1N1B4_4065 -1.8 -6.9 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf1N1B4_2280 -1.8 -8.1 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf1N1B4_1906 -1.8 -4.5 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) compare
Pf1N1B4_327 -1.7 -3.4 hypothetical protein compare
Pf1N1B4_5666 -1.7 -3.1 Oxidoreductase probably involved in sulfite reduction compare
Pf1N1B4_762 -1.7 -4.8 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf1N1B4_4299 -1.7 -3.4 sensor histidine kinase compare
Pf1N1B4_2269 -1.7 -3.3 Purine nucleoside phosphorylase (EC 2.4.2.1) compare
Pf1N1B4_1566 -1.7 -6.8 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf1N1B4_2208 -1.7 -6.7 Polyphosphate kinase (EC 2.7.4.1) compare
Pf1N1B4_932 -1.6 -2.9 FIG00963370: hypothetical protein compare
Pf1N1B4_2009 -1.6 -4.6 LysR family transcriptional regulator PA5437 compare
Pf1N1B4_578 -1.6 -3.6 enoyl-CoA hydratase, R-specific compare
Pf1N1B4_1535 -1.5 -2.9 hypothetical protein compare
Pf1N1B4_4518 -1.5 -2.6 Possible pyrimidine permease in reductive pathway compare
Pf1N1B4_1057 -1.4 -3.4 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf1N1B4_4723 -1.4 -2.7 UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide compare
Pf1N1B4_1617 -1.4 -6.1 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) compare
Pf1N1B4_716 -1.3 -3.6 Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites compare
Pf1N1B4_1239 -1.3 -6.0 Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18) conserved
Pf1N1B4_1263 -1.3 -2.5 Cu(I)-responsive transcriptional regulator compare
Pf1N1B4_4667 -1.3 -3.0 Acriflavin resistance plasma membrane protein compare
Pf1N1B4_2225 -1.2 -8.0 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
Pf1N1B4_5074 -1.2 -2.6 Gluconate permease compare
Pf1N1B4_3697 -1.2 -5.5 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf1N1B4_3508 -1.2 -3.9 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_799 -1.2 -2.5 hypothetical protein compare
Pf1N1B4_5675 -1.2 -2.8 hypothetical protein compare
Pf1N1B4_4678 -1.2 -2.5 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) compare
Pf1N1B4_3729 -1.2 -2.2 Quaternary ammonium compound-resistance protein SugE compare
Pf1N1B4_1619 -1.2 -6.6 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf1N1B4_4517 -1.1 -2.5 Dihydropyrimidinase (EC 3.5.2.2) compare
Pf1N1B4_1679 -1.1 -1.8 Membrane-fusion protein compare
Pf1N1B4_1391 -1.1 -4.2 Protein containing transglutaminase-like domain, putative cysteine protease compare
Pf1N1B4_1250 -1.1 -5.5 Nucleoside ABC transporter, periplasmic nucleoside-binding protein conserved
Pf1N1B4_5100 -1.1 -2.3 Transcriptional regulator ahyR/asaR family compare
Pf1N1B4_1 -1.1 -1.9 Colicin V production protein compare
Pf1N1B4_3613 -1.1 -1.8 hypothetical protein compare
Pf1N1B4_4494 -1.1 -2.6 hypothetical protein compare
Pf1N1B4_4419 -1.1 -1.9 Organosulfonate utilization protein SsuF compare
Pf1N1B4_2859 -1.1 -7.8 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf1N1B4_3991 -1.1 -1.3 Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family compare
Pf1N1B4_4515 -1.1 -2.5 Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2) compare
Pf1N1B4_715 -1.1 -4.0 Iron-regulated protein A precursor compare
Pf1N1B4_2004 -1.1 -2.1 putative membrane protein compare
Pf1N1B4_3143 -1.1 -2.6 RecA protein compare
Pf1N1B4_126 -1.1 -1.9 Pirin compare
Pf1N1B4_825 -1.1 -2.0 tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase compare
Pf1N1B4_5549 -1.1 -2.8 hypothetical protein compare
Pf1N1B4_4870 -1.0 -1.4 Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16) compare
Pf1N1B4_1653 -1.0 -4.7 Branched-chain amino acid transport protein AzlC compare
Pf1N1B4_5904 -1.0 -1.8 FIG00955320: hypothetical protein compare
Pf1N1B4_2144 -1.0 -5.5 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf1N1B4_4038 -1.0 -2.0 NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3) compare
Pf1N1B4_718 -1.0 -4.4 outer membrane component of uptake system, probably for ferrous iron (from data) compare
Pf1N1B4_4731 -1.0 -2.5 Sigma-54 dependent transcriptional regulator compare
Pf1N1B4_2917 -1.0 -5.0 FIG140336: TPR domain protein compare
Pf1N1B4_5346 -1.0 -2.7 hypothetical protein compare
Pf1N1B4_652 -1.0 -5.8 Sensory box histidine kinase compare
Pf1N1B4_2036 -1.0 -3.1 Phosphate transport system permease protein PstA (TC 3.A.1.7.1) compare
Pf1N1B4_5224 -1.0 -2.7 tRNA-Arg-CCT compare
Pf1N1B4_5645 -1.0 -2.6 Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2) compare
Pf1N1B4_2874 -1.0 -1.9 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf1N1B4_4839 -1.0 -1.8 lipoprotein compare
Pf1N1B4_338 -1.0 -2.6 Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-) compare
Pf1N1B4_2739 -1.0 -1.2 acetyltransferase, GNAT family compare
Pf1N1B4_3631 -1.0 -2.6 Flagellar motor rotation protein MotA compare
Pf1N1B4_3498 -1.0 -2.0 FIG00953240: hypothetical protein compare
Pf1N1B4_4810 -0.9 -2.5 Polymyxin resistance protein PmrM compare
Pf1N1B4_27 -0.9 -2.8 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf1N1B4_2005 -0.9 -1.8 ATP-dependent DNA helicase UvrD/PcrA compare
Pf1N1B4_2568 -0.9 -2.3 Translation elongation factor Tu compare
Pf1N1B4_959 -0.9 -7.4 Stringent starvation protein A compare
Pf1N1B4_4295 -0.9 -2.1 FIG00954434: hypothetical protein compare
Pf1N1B4_5829 -0.9 -1.9 FIG005107: hypothetical protein compare
Pf1N1B4_3713 -0.9 -1.5 Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21) compare
Pf1N1B4_3466 -0.9 -1.7 FIG00955605: hypothetical protein compare
Pf1N1B4_4592 -0.9 -1.7 Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1) compare
Pf1N1B4_4028 -0.9 -2.8 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) compare
Pf1N1B4_5077 -0.9 -1.9 D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) compare
Pf1N1B4_313 -0.9 -2.3 Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1) compare
Pf1N1B4_1462 -0.9 -6.9 Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase compare
Pf1N1B4_3290 -0.9 -1.4 Alternative cytochrome c oxidase polypeptide CoxP (EC 1.9.3.1) compare
Pf1N1B4_2037 -0.9 -2.4 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf1N1B4_106 -0.9 -1.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_5880 -0.9 -2.0 AttH component of AttEFGH ABC transport system compare
Pf1N1B4_4558 -0.9 -2.3 Outer membrane lipoprotein-sorting protein compare
Pf1N1B4_717 -0.9 -4.4 Iron-regulated protein A precursor compare
Pf1N1B4_4860 -0.9 -2.0 Sensory box sensor histidine kinase/response regulator (EC 2.7.3.-) compare
Pf1N1B4_1243 -0.9 -1.8 ABC transporter ATP-binding protein compare
Pf1N1B4_4402 -0.9 -1.9 SoxH protein, homolog compare
Pf1N1B4_3710 -0.9 -3.0 L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81) compare
Pf1N1B4_4990 -0.9 -1.6 FIG018171: hypothetical protein of Cupin superfamily compare
Pf1N1B4_4976 -0.9 -2.4 D-serine/D-alanine/glycine transporter compare
Pf1N1B4_751 -0.9 -1.3 hypothetical protein compare
Pf1N1B4_1568 -0.8 -2.6 Twin-arginine translocation protein TatB compare
Pf1N1B4_1236 -0.8 -3.4 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf1N1B4_5269 -0.8 -1.9 hypothetical protein compare
Pf1N1B4_5151 -0.8 -2.0 FIG002283: Isochorismatase family protein compare
Pf1N1B4_3368 -0.8 -1.6 Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) compare
Pf1N1B4_63 -0.8 -1.9 Phosphonoacetaldehyde phosphonohydrolase-related protein compare
Pf1N1B4_3730 -0.8 -2.1 Sodium-dependent transporter compare
Pf1N1B4_962 -0.8 -4.9 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf1N1B4_1967 -0.8 -2.2 hypothetical protein compare
Pf1N1B4_2612 -0.8 -2.3 Putative transport protein compare
Pf1N1B4_3906 -0.8 -1.9 DNA topoisomerase I (EC 5.99.1.2) compare
Pf1N1B4_2163 -0.8 -1.4 hypothetical protein compare
Pf1N1B4_4460 -0.8 -1.6 Cytochrome b561 compare
Pf1N1B4_5059 -0.8 -1.4 Transcriptional regulator, LysR family compare
Pf1N1B4_4474 -0.8 -1.7 transcription regulator protein compare
Pf1N1B4_1614 -0.8 -1.7 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf1N1B4_4936 -0.8 -3.0 hypothetical protein compare
Pf1N1B4_3413 -0.8 -1.0 Transcriptional regulator, LysR family compare
Pf1N1B4_3445 -0.8 -2.2 Succinylarginine dihydrolase (EC 3.5.3.23) compare
Pf1N1B4_3108 -0.8 -3.3 S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) compare
Pf1N1B4_5560 -0.8 -1.2 hypothetical protein compare
Pf1N1B4_4326 -0.8 -1.7 N-carbamoylputrescine amidase (3.5.1.53) compare
Pf1N1B4_5073 -0.8 -2.0 Hydroxypyruvate isomerase (EC 5.3.1.22) compare
Pf1N1B4_3152 -0.8 -3.2 LysR family transcriptional regulator PA3398 compare
Pf1N1B4_4481 -0.8 -1.4 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf1N1B4_4385 -0.8 -1.8 Gfa-like protein compare
Pf1N1B4_1554 -0.8 -3.6 predicted FeS cluster maintenance protein (from data) compare
Pf1N1B4_275 -0.8 -1.2 Organic hydroperoxide resistance transcriptional regulator compare
Pf1N1B4_3905 -0.8 -2.3 FIG00953423: hypothetical protein compare
Pf1N1B4_2538 -0.8 -3.2 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) compare
Pf1N1B4_4477 -0.8 -1.7 Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4) compare
Pf1N1B4_4673 -0.8 -1.8 4Fe-4S ferredoxin, iron-sulfur binding compare
Pf1N1B4_5084 -0.8 -2.0 Capsular polysaccharide synthesis enzyme CpsD, exopolysaccharide synthesis compare
Pf1N1B4_4603 -0.8 -1.6 hypothetical protein compare
Pf1N1B4_4799 -0.8 -1.3 Multidrug resistance protein B compare
Pf1N1B4_556 -0.8 -2.3 Gluconate permease compare
Pf1N1B4_1870 -0.8 -2.0 Zinc ABC transporter, ATP-binding protein ZnuC compare
Pf1N1B4_232 -0.8 -1.6 DNA-binding response regulator, LuxR family compare
Pf1N1B4_3234 -0.8 -2.2 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) compare
Pf1N1B4_2284 -0.8 -2.7 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf1N1B4_384 -0.7 -1.3 proteinase inhibitor compare
Pf1N1B4_5688 -0.7 -2.7 hypothetical protein compare
Pf1N1B4_4544 -0.7 -1.3 Uncharacterized protein ImpA compare
Pf1N1B4_5330 -0.7 -1.2 hypothetical protein compare
Pf1N1B4_4259 -0.7 -1.5 hypothetical protein compare
Pf1N1B4_4205 -0.7 -2.0 Probable signal peptide protein compare
Pf1N1B4_765 -0.7 -1.5 Cys-tRNA(Pro) deacylase YbaK compare
Pf1N1B4_1332 -0.7 -1.7 Cobalt-precorrin-6 synthase, anaerobic compare
Pf1N1B4_3588 -0.7 -1.6 N-Acetylneuraminate cytidylyltransferase (EC 2.7.7.43) compare
Pf1N1B4_5299 -0.7 -2.1 hypothetical protein compare
Pf1N1B4_1901 -0.7 -2.5 Putative NADH-flavin reductase compare


Specific Phenotypes

For 2 genes in this experiment

For nitrogen source Cytosine in Pseudomonas fluorescens FW300-N1B4

For nitrogen source Cytosine across organisms