Experiment set5IT008 for Azospirillum brasilense Sp245

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L-Malic acid carbon source and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days

Group: nitrogen fixation
Media: RCH2_defined_noCarbon_noNitrogen_noNitrilotriacetic_acid + L-Malic acid (20 mM)
Culturing: AzoBra_ML2a, serum bottle, Anaerobic, at 30 (C), shaken=0 rpm
Growth: about 3.1 generations
By: Kelly Wetmore; Jordan Baker on 26-Mar-19
Media components: 0.1 g/L Potassium Chloride, 0.49 g/L Sodium phosphate monobasic monohydrate, 0.97 g/L Potassium phosphate dibasic, Wolfe's mineral mix_minus_Nitrilotriacetic_acid (0.03 g/L Magnesium Sulfate Heptahydrate, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 16 genes in this experiment

For nitrogen fixation L-Malic acid in Azospirillum brasilense Sp245

For nitrogen fixation L-Malic acid across organisms

SEED Subsystems

Subsystem #Specific
Nitrogen fixation 8
Acetyl-CoA fermentation to Butyrate 2
Cobalt-zinc-cadmium resistance 1
Lipid A-Ara4N pathway ( Polymyxin resistance ) 1
Phosphate metabolism 1
Queuosine-Archaeosine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
nitrogen fixation I (ferredoxin) 1 1 1
nitrogen fixation II (flavodoxin) 1 1 1