Experiment set5IT007 for Pseudomonas fluorescens FW300-N1B4
Cytidine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_noNitrogen + Cytidine (5 mM), pH=7
Culturing: pseudo1_N1B4_ML1, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/16/2014
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Nplate1 C6
Specific Phenotypes
For 5 genes in this experiment
For nitrogen source Cytidine in Pseudomonas fluorescens FW300-N1B4
For nitrogen source Cytidine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cysteine Biosynthesis | 1 |
| Methionine Biosynthesis | 1 |
| Polyamine Metabolism | 1 |
| Pyrimidine utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| thiosulfate disproportionation IV (rhodanese) | 1 | 1 | 1 |
| L-cysteine biosynthesis I | 2 | 2 | 1 |
| L-cysteine degradation III | 2 | 1 | 1 |
| sulfide oxidation IV (mitochondria) | 5 | 3 | 1 |
| seleno-amino acid biosynthesis (plants) | 5 | 3 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| superpathway of seleno-compound metabolism | 19 | 6 | 1 |