Experiment set5IT007 for Bifidobacterium breve UCC2003

Recovery broth with Sulfamethizole 0.01 mM and Trimethoprim 0.003 mM

Group: stress
Media: Recovery broth + Sulfamethizole (0.01 mM) + Trimethoprim (0.003 mM), pH=6.8
Culturing: Bifido_ML2, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Anthony Shiver on 2/15/2020

Specific Phenotypes

For 2 genes in this experiment

For stress Sulfamethizole in Bifidobacterium breve UCC2003

For stress Sulfamethizole across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-adenosyl-L-methionine salvage II	3	2	1
S-adenosyl-L-methionine salvage I	4	4	1
L-methionine biosynthesis III	4	2	1
L-methionine biosynthesis I	5	3	1
seleno-amino acid biosynthesis (plants)	5	2	1
L-methionine biosynthesis II	6	5	1
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	6	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
superpathway of seleno-compound metabolism	19	4	1
aspartate superpathway	25	23	1