Experiment set5IT002 for Dechlorosoma suillum PS

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ALP with Nickel (II) chloride hexahydrate 2.5 mM

Group: stress
Media: ALP_richB + Nickel (II) chloride hexahydrate (2.5 mM)
Culturing: PS_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 4/20/2015
Media components: 0.97 g/L Sodium phosphate dibasic heptahydrate, 0.1 g/L Potassium Chloride, 0.25 g/L Ammonium chloride, 40 mM Sodium D,L-Lactate, 1.1 g/L Sodium pyruvate, 0.8203 g/L Sodium acetate, 2 g/L Yeast Extract, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1212 A3,A4

Specific Phenotypes

For 15 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Dechlorosoma suillum PS

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Ribonucleotide reduction 2
Ammonia assimilation 1
Arsenic resistance 1
Choline and Betaine Uptake and Betaine Biosynthesis 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Methionine Biosynthesis 1
Phosphate metabolism 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosine deoxyribonucleotides de novo biosynthesis I 2 2 1
L-threonine biosynthesis 2 2 1
guanosine deoxyribonucleotides de novo biosynthesis I 2 2 1
guanosine deoxyribonucleotides de novo biosynthesis II 4 3 2
adenosine deoxyribonucleotides de novo biosynthesis II 4 3 2
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 12 4
superpathway of adenosine nucleotides de novo biosynthesis II 7 6 2
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 2
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 9 2
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 8 2
superpathway of adenosine nucleotides de novo biosynthesis I 5 5 1
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 1
superpathway of L-threonine biosynthesis 6 6 1
L-methionine biosynthesis II 6 4 1
superpathway of purine nucleotides de novo biosynthesis II 26 24 4
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 6 1
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 6 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 18 2
superpathway of purine nucleotides de novo biosynthesis I 21 21 2
superpathway of histidine, purine, and pyrimidine biosynthesis 46 44 4
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of purine nucleotide salvage 14 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 12 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 15 1
aspartate superpathway 25 22 1