Experiment set5IT001 for Dechlorosoma suillum PS

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ALP with Nickel (II) chloride hexahydrate 1.5 mM

Group: stress
Media: ALP_richB + Nickel (II) chloride hexahydrate (1.5 mM)
Culturing: PS_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 4/20/2015
Media components: 0.97 g/L Sodium phosphate dibasic heptahydrate, 0.1 g/L Potassium Chloride, 0.25 g/L Ammonium chloride, 40 mM Sodium D,L-Lactate, 1.1 g/L Sodium pyruvate, 0.8203 g/L Sodium acetate, 2 g/L Yeast Extract, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1212 A1,A2

Specific Phenotypes

For 6 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Dechlorosoma suillum PS

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Ribonucleotide reduction 2
Methylcitrate cycle 1
Propionate-CoA to Succinate Module 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosine deoxyribonucleotides de novo biosynthesis I 2 2 1
guanosine deoxyribonucleotides de novo biosynthesis I 2 2 1
guanosine deoxyribonucleotides de novo biosynthesis II 4 3 2
adenosine deoxyribonucleotides de novo biosynthesis II 4 3 2
acrylate degradation II 3 1 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 12 4
superpathway of adenosine nucleotides de novo biosynthesis II 7 6 2
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 2
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 9 2
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 8 2
superpathway of adenosine nucleotides de novo biosynthesis I 5 5 1
2-methylcitrate cycle I 5 1 1
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 1
L-isoleucine biosynthesis IV 6 4 1
β-alanine biosynthesis II 6 1 1
2-methylcitrate cycle II 6 1 1
superpathway of purine nucleotides de novo biosynthesis II 26 24 4
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 6 1
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 6 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 18 2
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 2
superpathway of histidine, purine, and pyrimidine biosynthesis 46 44 4
superpathway of purine nucleotide salvage 14 13 1