Experiment set5H41 for Shewanella oneidensis MR-1

LB with Benzalkonium Chloride 0.004 mg/ml

Group: stress
Media: LB + Benzalkonium Chloride (0.004 mg/ml)
Culturing: MR1_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 8/21/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 653 C5,C6

Specific Phenotypes

For 19 genes in this experiment

For stress Benzalkonium Chloride in Shewanella oneidensis MR-1

For stress Benzalkonium Chloride across organisms

SEED Subsystems

Subsystem	#Specific
Formate hydrogenase	2
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
Cobalt-zinc-cadmium resistance	1
Mannose-sensitive hemagglutinin type 4 pilus	1
Molybdenum cofactor biosynthesis	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	1	1
putrescine biosynthesis I	2	1	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	1
putrescine biosynthesis II	3	3	1
spermidine biosynthesis III	4	2	1
superpathway of putrescine biosynthesis	4	2	1
molybdopterin biosynthesis	6	5	1
superpathway of polyamine biosynthesis II	8	6	1
superpathway of polyamine biosynthesis I	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	6	1
superpathway of L-arginine and L-ornithine degradation	13	8	1
superpathway of arginine and polyamine biosynthesis	17	13	1