Experiment set5H38 for Shewanella oneidensis MR-1

LB with Cisplatin 0.004 mg/ml

Group: stress
Media: LB + Cisplatin (0.004 mg/ml)
Culturing: MR1_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 8/21/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 653 B7,B8

Specific Phenotypes

For 95 genes in this experiment

For stress Cisplatin in Shewanella oneidensis MR-1

For stress Cisplatin across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	4
DNA repair, UvrABC system	2
DNA repair, bacterial	2
DNA repair, bacterial RecFOR pathway	2
Phage capsid proteins	2
Ammonia assimilation	1
DNA Repair Base Excision	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Glutamate metabolism
• Methionine metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamine degradation II	1	1	1
ammonia assimilation cycle III	3	3	2
L-glutamate and L-glutamine biosynthesis	7	6	2
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1