Experiment set5H37 for Phaeobacter inhibens DSM 17395

D-Sorbitol carbon source

Group: carbon source
Media: DinoMM_noCarbon_HighNutrient + D-Sorbitol (20 mM), pH=7
Culturing: Phaeo_ML1, tube, Aerobic, at 25 (C), shaken=200 rpm
Growth: about 4.6 generations
By: Adam on marchapr14
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 18 genes in this experiment

For carbon source D-Sorbitol in Phaeobacter inhibens DSM 17395

For carbon source D-Sorbitol across organisms

SEED Subsystems

Subsystem	#Specific
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	6
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Inositol catabolism	2
Fructose utilization	1
Mannitol Utilization	1
Phosphate metabolism	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Pentose and glucuronate interconversions
• Galactose metabolism
• Ascorbate and aldarate metabolism
• C21-Steroid hormone metabolism
• Puromycin biosynthesis
• Glycine, serine and threonine metabolism
• Bisphenol A degradation
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Inositol phosphate metabolism
• Linoleic acid metabolism
• alpha-Linolenic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Trinitrotoluene degradation
• Butanoate metabolism
• Retinol metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of ansamycins
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-sorbitol degradation I	3	3	1
myo-inositol degradation I	7	7	2
D-altritol and galactitol degradation	4	1	1
myo-, chiro- and scyllo-inositol degradation	10	7	2
mannitol cycle	5	3	1