Experiment set5H19 for Shewanella amazonensis SB2B

LB with Sodium Chloride 700 mM

Group: stress
Media: LB + Sodium Chloride (700 mM)
Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Adam on 7/30/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 594 C5,C6

Specific Phenotypes

For 17 genes in this experiment

For stress Sodium Chloride in Shewanella amazonensis SB2B

For stress Sodium Chloride across organisms

SEED Subsystems

Subsystem	#Specific
Formate hydrogenase	2
Anaerobic respiratory reductases	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
Experimental tye	1
Flavodoxin	1
Heme and Siroheme Biosynthesis	1
Mannose Metabolism	1
Molybdenum cofactor biosynthesis	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tryptophan metabolism
• Methane metabolism
• Porphyrin and chlorophyll metabolism
• Biosynthesis of alkaloids derived from shikimate pathway
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• Nicotinate and nicotinamide metabolism
• Limonene and pinene degradation
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superoxide radicals degradation	2	2	1
methanol oxidation to formaldehyde IV	2	1	1
ethanol degradation IV	3	3	1
NAD salvage pathway III (to nicotinamide riboside)	3	2	1
UTP and CTP dephosphorylation I	7	5	2
purine nucleotides degradation II (aerobic)	11	8	3
reactive oxygen species degradation	4	4	1
heme b biosynthesis I (aerobic)	4	4	1
inosine 5'-phosphate degradation	4	3	1
guanosine nucleotides degradation III	4	2	1
adenosine nucleotides degradation I	8	3	2
purine nucleotides degradation I (plants)	12	4	3
guanosine nucleotides degradation II	4	1	1
guanosine nucleotides degradation I	4	1	1
adenosine nucleotides degradation II	5	3	1
molybdopterin biosynthesis	6	5	1
superpathway of purines degradation in plants	18	4	3
superpathway of guanosine nucleotides degradation (plants)	6	1	1
ureide biosynthesis	7	3	1
superpathway of heme b biosynthesis from glycine	8	7	1
3,8-divinyl-chlorophyllide a biosynthesis III (aerobic, light independent)	9	3	1
3,8-divinyl-chlorophyllide a biosynthesis I (aerobic, light-dependent)	9	3	1
tunicamycin biosynthesis	9	1	1
superpathway of heme b biosynthesis from glutamate	10	10	1
NAD salvage (plants)	11	3	1