Experiment set5H16 for Shewanella amazonensis SB2B

LB with Cisplatin 0.01 mg/ml

Group: stress
Media: LB + Cisplatin (0.01 mg/ml)
Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Adam on 7/30/2013
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 594 B5,B6

Specific Phenotypes

For 24 genes in this experiment

For stress Cisplatin in Shewanella amazonensis SB2B

For stress Cisplatin across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	6
DNA repair, bacterial	4
DNA Repair Base Excision	3
DNA repair, UvrABC system	3
DNA repair, bacterial RecFOR pathway	3
Purine conversions	2
DNA repair, bacterial DinG and relatives	1
High affinity phosphate transporter and control of PHO regulon	1
Phosphate metabolism	1
Queuosine-Archaeosine Biosynthesis	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Oxidative phosphorylation
• Pyrimidine metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
guanine and guanosine salvage III	1	1	1
polyphosphate metabolism	2	2	1
superpathway of guanine and guanosine salvage	3	3	1
adenine and adenosine salvage V	3	3	1