Experiment set5H16 for Phaeobacter inhibens DSM 17395

Compare to:

Potassium acetate carbon source

Group: carbon source
Media: DinoMM_noCarbon_HighNutrient + Potassium acetate (20 mM), pH=7
Culturing: Phaeo_ML1, tube, Aerobic, at 25 (C), shaken=200 rpm
Growth: about 3.9 generations
By: Adam on marchapr14
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 8 genes in this experiment

For carbon source Potassium acetate in Phaeobacter inhibens DSM 17395

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Ketoisovalerate oxidoreductase 1
Photorespiration (oxidative C2 cycle) 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
ethylmalonyl-CoA pathway 11 7 4
superpathway of acetate utilization and formation 3 3 1
ethanol degradation IV 3 3 1
ethanol degradation II 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation III 3 2 1
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) 14 7 4
(2S)-ethylmalonyl-CoA biosynthesis 4 3 1
chitin deacetylation 4 2 1
2-methylcitrate cycle I 5 1 1
β-alanine biosynthesis II 6 4 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of the 3-hydroxypropanoate cycle 18 11 3
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
2-methylcitrate cycle II 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
methylaspartate cycle 19 15 3
glyoxylate assimilation 13 7 2
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 2 1
superpathway of coenzyme A biosynthesis II (plants) 10 8 1
3-hydroxypropanoate cycle 13 9 1
formaldehyde assimilation I (serine pathway) 13 8 1