Experiment set4S782 for Pseudomonas aeruginosa PA14

Compare to:

carbon source 10 mM 5-Aminovaleric acid

Group: carbon source
Media: Hans_Basal_Media + 5-Aminovaleric acid (10 mM), pH=7
Culturing: Paeruginosa_PA14_ML18, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 6/3/25
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 13 genes in this experiment

For carbon source 5-Aminovaleric acid in Pseudomonas aeruginosa PA14

For carbon source 5-Aminovaleric acid across organisms

SEED Subsystems

Subsystem #Specific
Polyamine Metabolism 6
Fatty Acid Biosynthesis FASII 1
mycolic acid synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
gondoate biosynthesis (anaerobic) 4 4 1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) 12 9 3
palmitate biosynthesis III 29 21 7
tetradecanoate biosynthesis (mitochondria) 25 17 6
palmitate biosynthesis II (type II fatty acid synthase) 31 29 7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 9 9 2
oleate biosynthesis IV (anaerobic) 14 13 3
superpathway of fatty acids biosynthesis (E. coli) 53 51 11
superpathway of unsaturated fatty acids biosynthesis (E. coli) 20 20 4
fatty acid elongation -- saturated 5 5 1
cis-vaccenate biosynthesis 5 5 1
8-amino-7-oxononanoate biosynthesis IV 5 4 1
L-lysine degradation IV 5 4 1
superpathway of fatty acid biosynthesis II (plant) 43 38 8
8-amino-7-oxononanoate biosynthesis I 11 9 2
anteiso-branched-chain fatty acid biosynthesis 34 24 6
even iso-branched-chain fatty acid biosynthesis 34 24 6
odd iso-branched-chain fatty acid biosynthesis 34 24 6
(5Z)-dodecenoate biosynthesis I 6 6 1
(5Z)-dodecenoate biosynthesis II 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
L-lysine degradation X 6 5 1
stearate biosynthesis IV 6 4 1
petroselinate biosynthesis 6 2 1
streptorubin B biosynthesis 34 20 5
biotin biosynthesis I 15 13 2
superpathway of fatty acid biosynthesis I (E. coli) 16 15 2
2-allylmalonyl-CoA biosynthesis 8 2 1
mycolate biosynthesis 205 20 5
superpathway of L-lysine degradation 43 15 1
superpathway of mycolate biosynthesis 239 21 5