Experiment set4S74 for Rhodopseudomonas palustris CGA009

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PM with 4-Hydroxybenzoic Acid carbon source

Group: carbon source
Media: PM + 4-Hydroxybenzoic Acid (3 mM) + Sodium bicarbonate (10 mM)
Culturing: RPal_CGA009_ML8, Anaerobic, at 30 (C), shaken=0 rpm
By: Yasu on 23-Mar-23
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium Sulfate, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source 4-Hydroxybenzoic Acid in Rhodopseudomonas palustris CGA009

For carbon source 4-Hydroxybenzoic Acid across organisms

SEED Subsystems

Subsystem #Specific
Benzoate transport and degradation cluster 2
Folate Biosynthesis 1
Protein chaperones 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
benzoate degradation II (aerobic and anaerobic) 1 1 1
NAD salvage pathway III (to nicotinamide riboside) 3 2 1
UTP and CTP dephosphorylation I 7 5 2
purine nucleotides degradation II (aerobic) 11 7 3
inosine 5'-phosphate degradation 4 3 1
guanosine nucleotides degradation II 4 3 1
guanosine nucleotides degradation III 4 3 1
adenosine nucleotides degradation I 8 5 2
purine nucleotides degradation I (plants) 12 7 3
guanosine nucleotides degradation I 4 2 1
chorismate biosynthesis from 3-dehydroquinate 5 5 1
adenosine nucleotides degradation II 5 3 1
superpathway of guanosine nucleotides degradation (plants) 6 3 1
superpathway of purines degradation in plants 18 8 3
chorismate biosynthesis I 7 7 1
ureide biosynthesis 7 3 1
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 1
tunicamycin biosynthesis 9 1 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
NAD salvage (plants) 11 4 1
chorismate biosynthesis II (archaea) 12 8 1
superpathway of L-tryptophan biosynthesis 13 13 1
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 7 1
superpathway of aromatic amino acid biosynthesis 18 18 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 8 1
superpathway of chorismate metabolism 59 40 1