Experiment set4S510 for Lysobacter sp. OAE881
Ying_OrganicAcid7 rep A; time point 2
Group: carbon sourceMedia: SDM_noCarbon + 1X Ying_OrganicAcid7
Culturing: Lysobacter_OAE881_ML4, tube, Aerobic, at 28 (C), shaken=180 rpm
By: Ying and Valentine on 1-Sep-23
Media components: 1.5 g/L Ammonium chloride, 0.6 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Ying_OrganicAcid7 1x includes: 366 uM Sodium L-Lactate, 366 uM D,L-Malic Acid, 366 uM Citric Acid, 366 uM Succinic Acid, 366 uM Sodium pyruvate, 366 uM a-Ketoglutaric acid disodium salt hydrate, 366 uM L-Citrulline
Specific Phenotypes
For 7 genes in this experiment
For carbon source Ying_OrganicAcid7 in Lysobacter sp. OAE881
For carbon source Ying_OrganicAcid7 across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| adipate degradation | 5 | 4 | 1 |
| fatty acid salvage | 6 | 5 | 1 |
| oleate β-oxidation | 35 | 27 | 3 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 9 | 1 |