Experiment set4S171 for Rahnella sp. WP5
NL-CCM; ambient oxygen
Group: nl-ccm; ambient oxygenMedia: NL-CCM
Culturing: RahnellaWP5_ML7, at 30 (C)
By: Doty lab on 1/12/24
Media components: 5 g/L Sucrose, 5 g/L D-Mannitol, 3.51 mM Sodium D,L-Lactate, 0.8 g/L Potassium phosphate dibasic, 0.2 g/L Potassium phosphate monobasic, 0.1 g/L Sodium Chloride, 0.025 g/L Sodium Molybdate Dihydrate, 0.028 g/L EDTA (disodium salt), 0.1 g/L Yeast Extract, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.06 g/L Calcium chloride dihydrate
Specific Phenotypes
For 18 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Butanol Biosynthesis | 1 |
| D-galactonate catabolism | 1 |
| Fermentations: Mixed acid | 1 |
| MazEF toxin-antitoxing (programmed cell death) system | 1 |
| Rrf2 family transcriptional regulators | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Puromycin biosynthesis
- Nucleotide sugars metabolism
- alpha-Linolenic acid metabolism
- Pyruvate metabolism
- Trinitrotoluene degradation
- Propanoate metabolism
- Butanoate metabolism
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| reductive monocarboxylic acid cycle | 2 | 2 | 1 |
| pyruvate fermentation to acetate IV | 3 | 3 | 1 |
| pyruvate fermentation to ethanol I | 3 | 3 | 1 |
| L-threonine degradation I | 6 | 6 | 1 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 9 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 8 | 1 |
| mixed acid fermentation | 16 | 16 | 1 |
| superpathway of L-threonine metabolism | 18 | 15 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 19 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 20 | 1 |