Experiment set4S144 for Rahnella sp. WP5

NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum

Group: mixed community
Media: NL-CCM + Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Culturing: RahnellaWP5_ML7, at 30 (C)
By: Doty lab on 1/12/24
Media components: 5 g/L Sucrose, 5 g/L D-Mannitol, 3.51 mM Sodium D,L-Lactate, 0.8 g/L Potassium phosphate dibasic, 0.2 g/L Potassium phosphate monobasic, 0.1 g/L Sodium Chloride, 0.025 g/L Sodium Molybdate Dihydrate, 0.028 g/L EDTA (disodium salt), 0.1 g/L Yeast Extract, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.06 g/L Calcium chloride dihydrate

Specific Phenotypes

For 1 genes in this experiment

For mixed community Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum in Rahnella sp. WP5

For mixed community Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-arginine degradation II (AST pathway)	5	5	1