Experiment set4S130 for Rahnella sp. WP5
NF_with_fullN
Group: nf_with_fullnMedia: NF_with_fullN
Culturing: RahnellaWP5_ML7, at 30 (C)
By: Doty lab on 1/12/24
Media components: 5 g/L Sucrose, 5 g/L D-Mannitol, 3.51 mM Sodium D,L-Lactate, 0.8 g/L Potassium phosphate dibasic, 0.2 g/L Potassium phosphate monobasic, 0.1 g/L Sodium Chloride, 0.025 g/L Sodium Molybdate Dihydrate, 0.028 g/L EDTA (disodium salt), 0.2 g/L Magnesium Sulfate Heptahydrate, 0.06 g/L Calcium chloride dihydrate, 1 g/L Ammonium chloride
Specific Phenotypes
For 4 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biotin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| cis-cyclopropane fatty acid (CFA) biosynthesis | 1 | 1 | 1 |
| biotin biosynthesis from 8-amino-7-oxononanoate I | 4 | 4 | 2 |
| biotin biosynthesis from 8-amino-7-oxononanoate II | 4 | 3 | 2 |
| sterculate biosynthesis | 2 | 1 | 1 |
| biotin biosynthesis from 8-amino-7-oxononanoate III | 5 | 3 | 2 |
| biotin biosynthesis II | 6 | 4 | 2 |
| biotin biosynthesis I | 15 | 12 | 2 |
| mycolate biosynthesis | 205 | 21 | 3 |
| superpathway of mycolate biosynthesis | 239 | 22 | 3 |