Experiment set4IT096 for Klebsiella michiganensis M5al

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Potassium acetate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Potassium acetate (20 mM), pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Mar-17
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1763 C4

Specific Phenotypes

For 8 genes in this experiment

For carbon source Potassium acetate in Klebsiella michiganensis M5al

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
Acetoin, butanediol metabolism 1
Branched-Chain Amino Acid Biosynthesis 1
Flavodoxin 1
Ketoisovalerate oxidoreductase 1
Photorespiration (oxidative C2 cycle) 1
Redox-dependent regulation of nucleus processes 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
pyruvate fermentation to (R)-acetoin II 2 2 1
glycolate and glyoxylate degradation II 2 2 1
chitin deacetylation 4 3 2
glyoxylate cycle 6 6 2
pyruvate fermentation to (S)-acetoin 3 3 1
ethanol degradation IV 3 3 1
pyruvate fermentation to (R)-acetoin I 3 3 1
superpathway of acetate utilization and formation 3 3 1
ethanol degradation II 3 3 1
L-isoleucine biosynthesis IV 6 4 2
ethanol degradation III 3 2 1
L-isoleucine biosynthesis V 3 2 1
L-valine biosynthesis 4 4 1
superpathway of (R,R)-butanediol biosynthesis 5 5 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
2-methylcitrate cycle I 5 2 1
superpathway of glyoxylate bypass and TCA 12 11 2
superpathway of 2,3-butanediol biosynthesis 6 5 1
2-methylcitrate cycle II 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
adlupulone and adhumulone biosynthesis 6 1 1
β-alanine biosynthesis II 6 1 1
lupulone and humulone biosynthesis 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
superpathway of glycol metabolism and degradation 7 7 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 2
L-isoleucine biosynthesis III 7 5 1
D-xylose degradation IV 7 3 1
L-arabinose degradation IV 8 4 1
L-isoleucine biosynthesis II 8 4 1
superpathway of branched chain amino acid biosynthesis 17 17 2
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 1 1
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 2
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) 14 2 1
superpathway of L-threonine metabolism 18 18 1
superpathway of pentose and pentitol degradation 42 21 1