Experiment set4IT089 for Pseudomonas fluorescens FW300-N2E3

LB with Benzalkonium Chloride 0.012 mg/ml

Group: stress
Media: LB + Benzalkonium Chloride (0.012 mg/ml)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 971 F5,F6

Specific Phenotypes

For 19 genes in this experiment

For stress Benzalkonium Chloride in Pseudomonas fluorescens FW300-N2E3

For stress Benzalkonium Chloride across organisms

SEED Subsystems

Subsystem	#Specific
Choline and Betaine Uptake and Betaine Biosynthesis	2
Multidrug Resistance Efflux Pumps	2
Oxidative stress	2
DNA-binding regulatory proteins, strays	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1
Ton and Tol transport systems	1
Two cell division clusters relating to chromosome partitioning	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Benzoxazinone biosynthesis
• Diterpenoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine betaine biosynthesis I (Gram-negative bacteria)	2	2	1
choline degradation I	2	2	1
glycine betaine biosynthesis II (Gram-positive bacteria)	2	2	1
choline-O-sulfate degradation	3	3	1