Experiment set4IT085 for Pseudomonas fluorescens FW300-N2E3
LB with Doxycycline hyclate 0.0002 mg/ml
Group: stressMedia: LB + Doxycycline hyclate (2e-04 mg/ml)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 971 D1,D2
Specific Phenotypes
For 8 genes in this experiment
For stress Doxycycline hyclate in Pseudomonas fluorescens FW300-N2E3
For stress Doxycycline hyclate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA repair, bacterial | 1 |
| Oxidative stress | 1 |
| Photorespiration (oxidative C2 cycle) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tryptophan metabolism
- Benzoxazinone biosynthesis
- Ubiquinone and menaquinone biosynthesis
- Glycine, serine and threonine metabolism
- Histidine metabolism
- Tyrosine metabolism
- Naphthalene and anthracene degradation
- Methane metabolism
- Porphyrin and chlorophyll metabolism
- Diterpenoid biosynthesis
- Carotenoid biosynthesis - General
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| superoxide radicals degradation | 2 | 2 | 1 |
| methanol oxidation to formaldehyde IV | 2 | 1 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| reactive oxygen species degradation | 4 | 4 | 1 |