Experiment set4IT084 for Pseudomonas fluorescens FW300-N1B4

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D-Mannose carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Mannose (20 mM), pH=7
Culturing: pseudo1_N1B4_ML1, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/16/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Cplate2 B4

Specific Phenotypes

For 5 genes in this experiment

For carbon source D-Mannose in Pseudomonas fluorescens FW300-N1B4

For carbon source D-Mannose across organisms

SEED Subsystems

Subsystem #Specific
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 2
D-Tagatose and Galactitol Utilization 1
Mannitol Utilization 1
Sialic Acid Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
methylglyoxal degradation III 2 1 1
N-acetylneuraminate and N-acetylmannosamine degradation II 3 1 1
D-fructuronate degradation 4 3 1
sulfoquinovose degradation III 4 1 1
mannitol cycle 5 4 1
sulfoquinovose degradation I 5 1 1
sulfoquinovose degradation VI 5 1 1
superpathway of β-D-glucuronosides degradation 7 4 1
sulfoquinovose degradation V 7 1 1
superpathway of methylglyoxal degradation 8 4 1
superpathway of hexuronide and hexuronate degradation 10 4 1
detoxification of reactive carbonyls in chloroplasts 10 4 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 14 1