Experiment set4IT084 for Acidovorax sp. GW101-3H11

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L-tyrosine disodium salt nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-tyrosine disodium salt (20 mM), pH=7
Culturing: acidovorax_3H11_ML3a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 6.4 generations
By: Mark on 6/10/2015
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Nplate1 D1

Specific Phenotypes

For 37 genes in this experiment

For nitrogen source L-tyrosine disodium salt in Acidovorax sp. GW101-3H11

For nitrogen source L-tyrosine disodium salt across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 5
Formate hydrogenase 3
ABC transporter oligopeptide (TC 3.A.1.5.1) 2
Glycine and Serine Utilization 2
Glycine cleavage system 2
Photorespiration (oxidative C2 cycle) 2
Acetyl-CoA fermentation to Butyrate 1
Ammonia assimilation 1
Aromatic amino acid degradation 1
Butanol Biosynthesis 1
Homogentisate pathway of aromatic compound degradation 1
Isoleucine degradation 1
Peptidyl-prolyl cis-trans isomerase 1
Periplasmic disulfide interchange 1
Plastoquinone Biosynthesis 1
Polyhydroxybutyrate metabolism 1
Potassium homeostasis 1
Serine-glyoxylate cycle 1
Tocopherol Biosynthesis 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
benzoyl-CoA biosynthesis 3 3 3
bis(guanylyl molybdenum cofactor) biosynthesis 2 2 2
bis(guanylyl tungstenpterin) cofactor biosynthesis 1 1 1
fatty acid β-oxidation III (unsaturated, odd number) 1 1 1
guanylyl molybdenum cofactor biosynthesis 1 1 1
formate oxidation to CO2 1 1 1
oleate β-oxidation 35 29 24
glutaryl-CoA degradation 5 3 3
fatty acid β-oxidation II (plant peroxisome) 5 3 3
2-methyl-branched fatty acid β-oxidation 14 10 8
fatty acid β-oxidation I (generic) 7 5 4
valproate β-oxidation 9 7 5
pyruvate fermentation to hexanol (engineered) 11 7 6
acetoacetate degradation (to acetyl CoA) 2 2 1
propanoate fermentation to 2-methylbutanoate 6 5 3
fatty acid salvage 6 5 3
pyruvate fermentation to butanol II (engineered) 6 5 3
L-isoleucine degradation I 6 5 3
oleate β-oxidation (thioesterase-dependent, yeast) 2 1 1
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 6 5
pyruvate fermentation to butanoate 7 3 3
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 3
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
4-hydroxybenzoate biosynthesis III (plants) 5 4 2
5,6-dehydrokavain biosynthesis (engineered) 10 6 4
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 3 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 2
L-tyrosine degradation I 5 3 2
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 4
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 2 2
pyruvate fermentation to butanol I 8 4 3
glycine biosynthesis II 3 3 1
ketolysis 3 3 1
glycine cleavage 3 3 1
polyhydroxybutanoate biosynthesis 3 3 1
methyl ketone biosynthesis (engineered) 6 3 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 3
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 4 3
oleate β-oxidation (reductase-dependent, yeast) 3 1 1
plastoquinol-9 biosynthesis I 3 1 1
L-glutamate degradation V (via hydroxyglutarate) 10 6 3
3-phenylpropanoate degradation 10 6 3
benzoyl-CoA degradation I (aerobic) 7 6 2
superpathway of glyoxylate cycle and fatty acid degradation 14 11 4
L-valine degradation I 8 6 2
(2S)-ethylmalonyl-CoA biosynthesis 4 2 1
L-glutamate degradation VII (to butanoate) 12 3 3
2-methylpropene degradation 8 2 2
oxalate degradation VI 4 1 1
oleate β-oxidation (isomerase-dependent, yeast) 4 1 1
4-hydroxy-2-nonenal detoxification 4 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 6 3
phenylacetate degradation I (aerobic) 9 5 2
ketogenesis 5 3 1
L-tryptophan degradation III (eukaryotic) 15 7 3
pentachlorophenol degradation 10 4 2
pyruvate fermentation to acetone 5 2 1
superpathway of plastoquinol biosynthesis 5 2 1
androstenedione degradation I (aerobic) 25 6 5
methyl tert-butyl ether degradation 10 2 2
isopropanol biosynthesis (engineered) 5 1 1
ethylbenzene degradation (anaerobic) 5 1 1
fatty acid β-oxidation VII (yeast peroxisome) 5 1 1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 1 1
oxalate degradation III 5 1 1
glycerol degradation to butanol 16 10 3
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 4 3
superpathway of phenylethylamine degradation 11 6 2
superpathway of testosterone and androsterone degradation 28 6 5
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 3
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 5 3
glyoxylate cycle 6 6 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 3
6-gingerol analog biosynthesis (engineered) 6 3 1
molybdopterin biosynthesis 6 2 1
toluene degradation VI (anaerobic) 18 4 3
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 7
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 2
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 2
4-ethylphenol degradation (anaerobic) 6 1 1
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) 6 1 1
jasmonic acid biosynthesis 19 5 3
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 2
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 8 7
androstenedione degradation II (anaerobic) 27 4 4
acetyl-CoA fermentation to butanoate 7 4 1
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 2
mevalonate pathway II (haloarchaea) 7 1 1
mevalonate pathway I (eukaryotes and bacteria) 7 1 1
vitamin E biosynthesis (tocopherols) 7 1 1
Spodoptera littoralis pheromone biosynthesis 22 4 3
glutathione-mediated detoxification I 8 3 1
2-deoxy-D-ribose degradation II 8 3 1
mevalonate pathway III (Thermoplasma) 8 1 1
mevalonate pathway IV (archaea) 8 1 1
isoprene biosynthesis II (engineered) 8 1 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 2
platensimycin biosynthesis 26 7 3
1-butanol autotrophic biosynthesis (engineered) 27 19 3
L-phenylalanine degradation IV (mammalian, via side chain) 9 4 1
4-oxopentanoate degradation 9 2 1
glutathione-mediated detoxification II 9 1 1
gliotoxin biosynthesis 9 1 1
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) 10 4 1
L-lysine fermentation to acetate and butanoate 10 2 1
gallate degradation III (anaerobic) 11 4 1
ethylmalonyl-CoA pathway 11 3 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 2 2
superpathway of glyoxylate bypass and TCA 12 10 1
superpathway of C1 compounds oxidation to CO2 12 4 1
indole glucosinolate activation (intact plant cell) 12 3 1
camalexin biosynthesis 12 2 1
superpathway of cholesterol degradation III (oxidase) 49 4 4
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 2
purine nucleobases degradation I (anaerobic) 15 6 1
sitosterol degradation to androstenedione 18 1 1
purine nucleobases degradation II (anaerobic) 24 15 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 1
superpathway of ergosterol biosynthesis I 26 5 1
superpathway of cholesterol biosynthesis 38 5 1
superpathway of L-lysine degradation 43 10 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 19 1