Experiment set4IT078 for Pseudomonas fluorescens FW300-N2E3

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LB with 1-ethyl-3-methylimidazolium chloride 170 mM

Group: stress
Media: LB + 1-ethyl-3-methylimidazolium chloride (170 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 970 D7,D8

Specific Phenotypes

For 9 genes in this experiment

For stress 1-ethyl-3-methylimidazolium chloride in Pseudomonas fluorescens FW300-N2E3

For stress 1-ethyl-3-methylimidazolium chloride across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 3
Potassium homeostasis 2
Bacterial RNA-metabolizing Zn-dependent hydrolases 1
Carboxysome 1
Conserved gene cluster associated with Met-tRNA formyltransferase 1
DNA-binding regulatory proteins, strays 1
Glutathione-regulated potassium-efflux system and associated functions 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
NADH to cytochrome bo oxidase electron transfer I 2 2 1
NADH to cytochrome bo oxidase electron transfer II 2 2 1
NADH to nitrate electron transfer 2 2 1
nitrate reduction VIIIb (dissimilatory) 2 1 1
NADH to cytochrome bd oxidase electron transfer I 2 1 1
NADH to cytochrome bd oxidase electron transfer II 2 1 1
NADH to cytochrome aa3 oxidase electron transfer 2 1 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
mitochondrial NADPH production (yeast) 5 4 1
Fe(II) oxidation 6 3 1
NAD(P)/NADPH interconversion 6 3 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 6 1