Experiment set4IT078 for Synechococcus elongatus PCC 7942

D-Cycloserine 0.0625 mg/ml

Group: stress
Media: BG11 + D-Cycloserine (0.0625 mg/ml)
Culturing: SynE_ML6, 12 well microplate, Aerobic, at 30 (C), shaken=0 rpm
Growth: about 3.4 generations
By: Kelly on 6/20/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 12 genes in this experiment

For stress D-Cycloserine in Synechococcus elongatus PCC 7942

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem	#Specific
Glycolate, glyoxylate interconversions	2
Photorespiration (oxidative C2 cycle)	2
Methionine Salvage	1
NAD and NADP cofactor biosynthesis global	1
Pyruvate Alanine Serine Interconversions	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glyoxylate and dicarboxylate metabolism
• Methionine metabolism
• Taurine and hypotaurine metabolism
• Glycerolipid metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Nicotinate and nicotinamide metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine degradation IV	1	1	1
3-methylthiopropanoate biosynthesis	1	1	1
NADP biosynthesis	1	1	1
NAD phosphorylation and transhydrogenation	2	1	1
glycerol degradation II	2	1	1
glycerol degradation V	2	1	1
glycolate and glyoxylate degradation II	2	1	1
glycolate and glyoxylate degradation III	3	1	1
NAD phosphorylation and dephosphorylation	3	1	1
glycolate and glyoxylate degradation I	4	2	1
superpathway of glycerol degradation to 1,3-propanediol	4	1	1
cytosolic NADPH production (yeast)	5	3	1
mitochondrial NADPH production (yeast)	5	2	1
NAD(P)/NADPH interconversion	6	3	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	5	1
superpathway of glycol metabolism and degradation	7	3	1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast)	8	4	1
photorespiration I	9	8	1
photorespiration III	9	8	1
photorespiration II	10	8	1
L-methionine salvage cycle III	11	9	1
L-methionine salvage cycle II (plants)	11	6	1
L-methionine salvage cycle I (bacteria and plants)	12	8	1
glycerol degradation to butanol	16	8	1