Experiment set4IT075 for Pseudomonas fluorescens FW300-N2E3

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LB with 1-ethyl-3-methylimidazolium acetate 15 mM

Group: stress
Media: LB + 1-ethyl-3-methylimidazolium acetate (15 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 970 D1,D2

Specific Phenotypes

For 6 genes in this experiment

For stress 1-ethyl-3-methylimidazolium acetate in Pseudomonas fluorescens FW300-N2E3

For stress 1-ethyl-3-methylimidazolium acetate across organisms

SEED Subsystems

Subsystem #Specific
Anaerobic respiratory reductases 1
Cysteine Biosynthesis 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Photorespiration (oxidative C2 cycle) 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
L-glutamate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
glycine cleavage 3 3 1
glycine biosynthesis II 3 3 1
molybdenum cofactor biosynthesis 3 1 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 4 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
guanosine ribonucleotides de novo biosynthesis 4 4 1
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 1
L-glutamate and L-glutamine biosynthesis 7 6 1
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 1
L-citrulline biosynthesis 8 7 1
superpathway of L-citrulline metabolism 12 9 1
superpathway of purine nucleotide salvage 14 13 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of purine nucleotides de novo biosynthesis II 26 24 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 44 1