Experiment set4IT074 for Pseudomonas fluorescens FW300-N1B4

Compare to:

L-Isoleucine carbon source

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_3115 +2.4 14.3 RNA polymerase sigma factor RpoS compare
Pf1N1B4_218 +1.8 9.9 Periplasmic binding protein compare
Pf1N1B4_688 +1.7 3.2 FIG00955483: hypothetical protein compare
Pf1N1B4_262 +1.4 12.5 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf1N1B4_4937 +1.4 4.6 Two-component hybrid sensor and regulator compare
Pf1N1B4_407 +1.3 1.9 ADP-ribosylglycohydrolase YegU (EC 3.2.-.-) compare
Pf1N1B4_2177 +1.3 1.7 FIG00953342: hypothetical protein compare
Pf1N1B4_4855 +1.2 2.6 Response regulator compare
Pf1N1B4_4687 +1.2 6.1 Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21) compare
Pf1N1B4_2442 +1.1 2.7 Electron transfer flavoprotein, beta subunit compare
Pf1N1B4_1489 +1.1 7.7 Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) conserved
Pf1N1B4_5870 +1.1 2.4 VgrG protein compare
Pf1N1B4_4103 +1.1 2.3 Flagellar synthesis regulator FleN compare
Pf1N1B4_1885 +1.1 6.4 alginate biosynthesis transcriptional regulatory protein AlgB compare
Pf1N1B4_3794 +1.1 2.7 hypothetical protein compare
Pf1N1B4_3911 +1.1 3.9 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf1N1B4_4826 +1.1 1.8 glutamine synthetase family protein compare
Pf1N1B4_3800 +1.1 2.3 Enoyl-CoA hydratase/isomerase family protein compare
Pf1N1B4_2224 +1.1 2.1 FIG00953390: hypothetical protein compare
Pf1N1B4_4822 +1.1 2.4 Paraquat-inducible protein A compare
Pf1N1B4_4295 +1.1 2.4 FIG00954434: hypothetical protein compare
Pf1N1B4_3514 +1.0 8.3 Sensor histidine kinase/response regulator compare
Pf1N1B4_3129 +1.0 1.8 Prophage baseplate assembly protein V compare
Pf1N1B4_695 +1.0 2.3 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf1N1B4_2357 +1.0 6.4 type IV pilus biogenesis protein PilJ compare
Pf1N1B4_4560 +1.0 2.0 Transcriptional regulator, MerR family compare
Pf1N1B4_5146 +1.0 1.8 Chromosome initiation inhibitor compare
Pf1N1B4_3764 +1.0 2.3 Allantoicase (EC 3.5.3.4) compare
Pf1N1B4_4755 +1.0 2.0 ribosomal protein S6 glutaminyl transferase related protein compare
Pf1N1B4_749 +1.0 1.8 hypothetical protein compare
Pf1N1B4_4874 +1.0 2.2 hypothetical protein compare
Pf1N1B4_4359 +1.0 2.6 PvcA protein, related to known isonitrile synthases compare
Pf1N1B4_5801 +1.0 2.6 hypothetical protein compare
Pf1N1B4_2322 +1.0 2.2 FIG015547: peptidase, M16 family compare
Pf1N1B4_761 +1.0 2.0 FIG00954879: hypothetical protein compare
Pf1N1B4_288 +1.0 7.0 FIG00958794: hypothetical protein compare
Pf1N1B4_1334 +1.0 1.6 FIG00955840: hypothetical protein compare
Pf1N1B4_290 +1.0 8.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_3991 +1.0 1.5 Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family compare
Pf1N1B4_1256 +0.9 2.8 Transcriptional regulator, LysR family compare
Pf1N1B4_4603 +0.9 1.6 hypothetical protein compare
Pf1N1B4_2102 +0.9 1.6 hypothetical protein compare
Pf1N1B4_1370 +0.9 1.4 Unsaturated fatty acid biosythesis repressor FabR, TetR family compare
Pf1N1B4_4982 +0.9 1.7 Transcriptional regulator, AraC family compare
Pf1N1B4_5512 +0.9 1.5 hypothetical protein compare
Pf1N1B4_2925 +0.9 2.4 hypothetical protein compare
Pf1N1B4_1555 +0.9 5.7 Polyhydroxyalkanoic acid synthase compare
Pf1N1B4_1756 +0.9 2.2 Aspartyl/asparaginyl beta-hydroxylase and related dioxygenases compare
Pf1N1B4_3605 +0.9 1.5 Flagellar motor switch protein FliG compare
Pf1N1B4_814 +0.9 2.2 Transcriptional regulator, PadR family compare
Pf1N1B4_4521 +0.9 2.3 Probable transmembrane protein compare
Pf1N1B4_2456 +0.9 2.1 Sarcosine oxidase delta subunit (EC 1.5.3.1) compare
Pf1N1B4_5435 +0.9 2.1 hypothetical protein compare
Pf1N1B4_5995 +0.9 1.6 Transporter, MFS superfamily compare
Pf1N1B4_4337 +0.9 1.8 Transcriptional regulator compare
Pf1N1B4_1008 +0.8 1.5 FIG006045: Sigma factor, ECF subfamily compare
Pf1N1B4_2169 +0.8 1.8 FIG00953808: hypothetical protein compare
Pf1N1B4_4818 +0.8 1.4 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf1N1B4_4532 +0.8 1.8 ClpB protein compare
Pf1N1B4_4087 +0.8 2.2 MISCELLANEOUS; Unknown compare
Pf1N1B4_5269 +0.8 1.6 hypothetical protein compare
Pf1N1B4_4980 +0.8 1.6 putative lipoprotein compare
Pf1N1B4_2071 +0.8 1.5 Protein phosphatase ImpM compare
Pf1N1B4_4349 +0.8 2.1 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) compare
Pf1N1B4_1679 +0.8 1.3 Membrane-fusion protein compare
Pf1N1B4_4402 +0.8 1.8 SoxH protein, homolog compare
Pf1N1B4_4812 +0.8 2.2 Site-specific recombinase, phage integrase family compare
Pf1N1B4_2635 +0.8 1.8 hypothetical protein compare
Pf1N1B4_1519 +0.8 2.3 3-oxoacyl-[ACP] reductase (EC 1.1.1.100) compare
Pf1N1B4_2382 +0.8 5.5 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_5668 +0.8 1.6 Peptidase, U7 family compare
Pf1N1B4_1721 +0.8 1.7 DNA-binding heavy metal response regulator compare
Pf1N1B4_5055 +0.8 2.3 (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-) compare
Pf1N1B4_5283 +0.8 1.8 hypothetical protein compare
Pf1N1B4_3446 +0.8 1.4 Putative GTPases (G3E family) compare
Pf1N1B4_5552 +0.8 1.7 hypothetical protein compare
Pf1N1B4_3516 +0.8 5.6 TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins compare
Pf1N1B4_3435 +0.8 1.5 ABC transporter for L-Arginine and L-Citrulline, ATPase component (from data) compare
Pf1N1B4_2484 +0.8 2.1 hypothetical protein compare
Pf1N1B4_5629 +0.8 2.8 Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1) compare
Pf1N1B4_5708 +0.8 2.1 Transcriptional regulator, TetR family compare
Pf1N1B4_5854 +0.8 3.7 FIG00960144: hypothetical protein compare
Pf1N1B4_3722 +0.8 1.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_5159 +0.8 2.3 Cellulose synthase operon protein C compare
Pf1N1B4_164 +0.8 1.3 ADP-ribosylglycohydrolase family protein compare
Pf1N1B4_68 +0.7 1.7 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) compare
Pf1N1B4_2178 +0.7 1.4 Regulator of nucleoside diphosphate kinase compare
Pf1N1B4_3515 +0.7 2.8 FIG00953467: hypothetical protein compare
Pf1N1B4_731 +0.7 1.9 Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like compare
Pf1N1B4_4768 +0.7 2.7 hypothetical protein compare
Pf1N1B4_2471 +0.7 2.5 putative iron-regulated membrane protein compare
Pf1N1B4_5803 +0.7 2.5 Catechol 1,2-dioxygenase (EC 1.13.11.1) compare
Pf1N1B4_1472 +0.7 1.4 Lipopolysaccharide heptosyltransferase I (EC 2.4.1.-) compare
Pf1N1B4_1005 +0.7 1.7 probable membrane protein YPO0899 compare
Pf1N1B4_3627 +0.7 1.2 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf1N1B4_2835 +0.7 2.6 hypothetical protein compare
Pf1N1B4_5575 +0.7 1.8 hypothetical protein compare
Pf1N1B4_4695 +0.7 1.4 hypothetical protein compare
Pf1N1B4_2356 +0.7 2.0 hypothetical protein compare
Pf1N1B4_5628 +0.7 2.0 Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1) compare
Pf1N1B4_3232 +0.7 2.5 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12) compare
Pf1N1B4_5447 +0.7 1.5 hypothetical protein compare
Pf1N1B4_526 +0.7 2.2 Undecaprenyl pyrophosphate synthase compare
Pf1N1B4_2145 +0.7 1.2 Translation initiation inhibitor compare
Pf1N1B4_4472 +0.7 1.7 Transcriptional regulator, ArsR family compare
Pf1N1B4_2433 +0.7 1.7 FIG00953108: hypothetical protein compare
Pf1N1B4_4590 +0.7 1.7 Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1) compare
Pf1N1B4_971 +0.7 5.9 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
Pf1N1B4_4810 +0.7 1.3 Polymyxin resistance protein PmrM compare
Pf1N1B4_103 +0.7 2.6 Histidine permease YuiF compare
Pf1N1B4_5327 +0.7 3.3 hypothetical protein compare
Pf1N1B4_1475 +0.7 2.9 FIG00962376: hypothetical protein compare
Pf1N1B4_291 +0.7 4.1 Major outer membrane lipoprotein I compare
Pf1N1B4_625 +0.7 2.3 FIG023103: Predicted transmembrane protein compare
Pf1N1B4_1293 +0.7 2.0 hypothetical protein compare
Pf1N1B4_675 +0.7 1.3 hypothetical protein compare
Pf1N1B4_4151 +0.7 2.8 FIG00960153: hypothetical protein compare
Pf1N1B4_1094 +0.7 1.5 FIG005080: Possible exported protein compare
Pf1N1B4_3938 +0.7 1.7 Integral membrane protein TerC compare
Pf1N1B4_3830 +0.7 2.6 CrfX protein compare
Pf1N1B4_2682 +0.7 1.3 Antiholin-like protein LrgA compare
Pf1N1B4_2949 +0.7 0.8 FIG00956396: hypothetical protein compare
Pf1N1B4_1403 +0.7 3.4 3'-to-5' exoribonuclease RNase R compare
Pf1N1B4_1346 +0.7 1.8 Urea ABC transporter, ATPase protein UrtD compare
Pf1N1B4_5015 +0.7 2.1 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf1N1B4_133 +0.7 1.9 Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis compare
Pf1N1B4_538 +0.7 1.4 hypothetical protein compare
Pf1N1B4_286 +0.7 4.9 2-dehydropantoate 2-reductase (EC 1.1.1.169) compare
Pf1N1B4_5814 +0.7 1.7 putative membrane protein compare
Pf1N1B4_4700 +0.7 1.1 FIG00956898: hypothetical protein compare
Pf1N1B4_969 +0.7 6.3 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf1N1B4_2211 +0.7 2.9 Putative amino acid ABC transporter, permease protein compare
Pf1N1B4_973 +0.7 5.8 Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-) compare
Pf1N1B4_628 +0.7 2.8 Queuosine Biosynthesis QueC ATPase compare
Pf1N1B4_1155 +0.7 2.1 hypothetical protein compare
Pf1N1B4_5886 +0.7 1.9 Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6) compare
Pf1N1B4_251 +0.7 2.4 DNA-binding response regulator, LuxR family compare
Pf1N1B4_5382 +0.6 2.2 hypothetical protein compare
Pf1N1B4_32 +0.6 1.7 Transcriptional regulator, TetR family compare
Pf1N1B4_5916 +0.6 1.5 Similar to non-heme chloroperoxidase, sll5080 homolog compare
Pf1N1B4_5598 +0.6 1.7 hypothetical protein compare
Pf1N1B4_5851 +0.6 1.6 Transcriptional regulator, RpiR family compare
Pf1N1B4_4568 +0.6 1.2 FIG00793915: hypothetical protein compare
Pf1N1B4_5858 +0.6 1.9 carboxyphosphonoenolpyruvate phosphonomutase, putative compare
Pf1N1B4_756 +0.6 1.3 hypothetical protein compare
Pf1N1B4_4341 +0.6 1.9 hypothetical protein compare
Pf1N1B4_773 +0.6 2.4 ABC transporter for L-asparagine and L-glutamate, permease subunit 2 (from data) compare
Pf1N1B4_2355 +0.6 2.0 type IV pili signal transduction protein PilI compare
Pf1N1B4_2035 +0.6 2.8 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf1N1B4_5554 +0.6 1.8 hypothetical protein compare
Pf1N1B4_3050 +0.6 2.1 FIG00958830: hypothetical protein compare
Pf1N1B4_998 +0.6 2.4 Membrane fusion component of tripartite multidrug resistance system compare
Pf1N1B4_4303 +0.6 1.7 DipZ protein compare
Pf1N1B4_5793 +0.6 1.6 Protein RtcB compare
Pf1N1B4_5109 +0.6 1.1 Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases compare
Pf1N1B4_2106 +0.6 1.5 Hydrogen peroxide-inducible genes activator compare
Pf1N1B4_4932 +0.6 1.8 Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83) compare
Pf1N1B4_4136 +0.6 1.4 hypothetical protein compare
Pf1N1B4_5607 +0.6 1.2 C4-dicarboxylate transport protein compare
Pf1N1B4_4118 +0.6 1.6 Cyn operon transcriptional activator compare
Pf1N1B4_3222 +0.6 1.0 Ferredoxin compare
Pf1N1B4_2544 +0.6 3.6 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf1N1B4_5763 +0.6 1.3 Nitric oxide reductase activation protein NorQ compare
Pf1N1B4_501 +0.6 1.0 Flp pilus assembly protein RcpC/CpaB compare
Pf1N1B4_4869 +0.6 1.7 Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16) compare
Pf1N1B4_1278 +0.6 1.5 Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily compare
Pf1N1B4_3382 +0.6 2.1 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262) compare
Pf1N1B4_2184 +0.6 3.2 Autolysis response regulater LytR compare
Pf1N1B4_4355 +0.6 1.3 Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-) compare
Pf1N1B4_5187 +0.6 0.9 5S RNA compare
Pf1N1B4_4528 +0.6 2.3 Predicted aminoglycoside phosphotransferase compare
Pf1N1B4_1466 +0.6 1.8 hypothetical protein compare
Pf1N1B4_5302 +0.6 1.5 hypothetical protein compare
Pf1N1B4_5542 +0.6 1.1 hypothetical protein compare
Pf1N1B4_4847 +0.6 1.3 Various polyols ABC transporter, ATP-binding component compare
Pf1N1B4_3831 +0.6 2.6 CmpX compare
Pf1N1B4_1612 +0.6 1.2 FIG00955429: hypothetical protein compare
Pf1N1B4_2441 +0.6 2.0 Electron transfer flavoprotein, alpha subunit compare
Pf1N1B4_5282 +0.6 1.5 Phage protein compare
Pf1N1B4_1139 +0.6 1.8 FOG: TPR repeat, SEL1 subfamily compare
Pf1N1B4_3350 +0.6 1.4 FIG00954807: hypothetical protein compare
Pf1N1B4_1335 +0.6 3.0 Copper metallochaperone, bacterial analog of Cox17 protein compare
Pf1N1B4_205 +0.6 1.7 Pyoverdin biosynthesis protein PvdH, L-2,4-diaminobutyrate:2-oxoglutarate aminotransferase (EC 2.6.1.76) compare
Pf1N1B4_4650 +0.6 1.7 Aspartate racemase (EC 5.1.1.13) compare
Pf1N1B4_972 +0.6 5.2 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf1N1B4_3918 +0.6 1.5 Thiamin biosynthesis lipoprotein ApbE compare
Pf1N1B4_1838 +0.6 1.6 FIG00955509: hypothetical protein compare
Pf1N1B4_5433 +0.6 1.6 hypothetical protein compare
Pf1N1B4_2214 +0.6 1.7 Transcriptional regulator, GntR family compare
Pf1N1B4_5353 +0.6 1.7 hypothetical protein compare
Pf1N1B4_4518 +0.6 1.2 Possible pyrimidine permease in reductive pathway compare
Pf1N1B4_2774 +0.6 1.5 hypothetical protein compare
Pf1N1B4_6031 +0.6 1.7 Ribokinase (EC 2.7.1.15) (from data) compare
Pf1N1B4_2851 +0.6 0.9 hypothetical protein compare
Pf1N1B4_6041 +0.6 0.9 Carbon storage regulator compare
Pf1N1B4_4133 +0.6 1.7 Mobile element protein compare
Pf1N1B4_608 +0.6 1.3 Heme oxygenase HemO, associated with heme uptake compare
Pf1N1B4_4256 +0.6 1.2 Probable transmembrane protein compare
Pf1N1B4_3275 +0.6 1.8 L-lysine permease compare
Pf1N1B4_3976 +0.6 2.8 Transmembrane regulator protein PrtR compare


Specific Phenotypes

For 6 genes in this experiment

For carbon source L-Isoleucine in Pseudomonas fluorescens FW300-N1B4

For carbon source L-Isoleucine across organisms