Experiment set4IT072 for Shewanella sp. ANA-3

LB with Tetracycline hydrochloride 0.001 mg/ml

Group: stress
Media: LB + Tetracycline hydrochloride (0.001 mg/ml)
Culturing: ANA3_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Mark on 1/14/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1092 E3,E4

Specific Phenotypes

For 7 genes in this experiment

For stress Tetracycline hydrochloride in Shewanella sp. ANA-3

For stress Tetracycline hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Ammonia assimilation	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
ZZ gjo need homes	1
cAMP signaling in bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Glutamate metabolism
• Glutathione metabolism
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamine degradation II	1	1	1
ammonia assimilation cycle III	3	3	2
L-glutamate and L-glutamine biosynthesis	7	6	2
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1