Experiment set4IT070 for Pseudomonas fluorescens FW300-N2E3

Compare to:

LB with Cholin acetate 38 mM

200 most detrimental genes:

  gene name fitness t score description  
AO353_02940 +3.1 7.3 DNA-binding protein compare
AO353_11530 +2.9 14.6 diguanylate cyclase compare
AO353_08835 +2.7 15.3 CDP-6-deoxy-delta-3,4-glucoseen reductase compare
AO353_04220 +2.4 9.2 chemotaxis protein CheY compare
AO353_10550 +2.0 4.8 glutamine amidotransferase compare
AO353_18775 +1.9 6.0 flagellar biosynthesis protein FlhF compare
AO353_11525 +1.8 6.3 aminotransferase compare
AO353_00115 +1.8 4.1 hypothetical protein compare
AO353_17625 +1.8 9.6 RNA polymerase subunit sigma compare
AO353_27710 +1.7 4.5 DNA repair protein compare
AO353_18285 +1.7 3.2 3-phosphoglycerate kinase compare
AO353_07475 +1.7 8.4 sulfurase compare
AO353_16495 +1.6 6.8 zinc metallopeptidase RseP compare
AO353_07480 +1.6 10.7 LysR family transcriptional regulator compare
AO353_09115 +1.5 8.6 diguanylate cyclase compare
AO353_10545 +1.5 10.6 ferredoxin compare
AO353_07470 +1.5 9.9 acetylornithine aminotransferase compare
AO353_02195 +1.5 4.1 spermidine synthase compare
AO353_22500 +1.4 2.9 hypothetical protein compare
AO353_10540 +1.4 11.6 aminobenzoate oxygenase compare
AO353_08730 +1.4 7.1 glycine cleavage system protein H compare
AO353_03605 +1.3 1.9 FmdB family transcriptional regulator compare
AO353_00700 +1.3 5.5 Cro/Cl family transcriptional regulator compare
AO353_00120 +1.2 3.4 DNA-binding protein compare
AO353_07515 +1.2 1.5 transporter compare
AO353_01375 +1.2 4.3 phosphate acyltransferase compare
AO353_12850 +1.2 9.5 diguanylate cyclase compare
AO353_06015 +1.1 3.8 hypothetical protein compare
AO353_11540 +1.1 4.9 ABC transporter permease compare
AO353_10535 +1.1 6.7 MFS transporter compare
AO353_16440 +1.1 3.6 ArsC family transcriptional regulator compare
AO353_10520 +1.0 3.5 GntR family transcriptional regulator compare
AO353_02175 +1.0 1.9 carbon storage regulator compare
AO353_10495 +1.0 3.9 serine hydroxymethyltransferase compare
AO353_27160 +1.0 3.0 Pathogenicity locus compare
AO353_19085 +1.0 2.6 organic hydroperoxide resistance protein compare
AO353_23910 +1.0 2.5 response regulator compare
AO353_00835 +0.9 4.0 transporter compare
AO353_00195 +0.9 2.3 virion morphogenesis protein compare
AO353_14385 +0.9 3.5 3-methyl-2-oxobutanoate hydroxymethyltransferase compare
AO353_14915 +0.9 2.8 (2Fe-2S)-binding protein compare
AO353_18325 +0.9 2.5 hypothetical protein compare
AO353_12145 +0.9 3.9 nitrogen regulation protein NR(I) compare
AO353_27350 +0.9 2.8 hypothetical protein compare
AO353_27855 +0.9 1.4 MarR family transcriptional regulator compare
AO353_04055 +0.9 1.4 methionine--tRNA ligase compare
AO353_26285 +0.9 1.9 hypothetical protein compare
AO353_13130 +0.9 2.5 N-acetylmuramoyl-L-alanine amidase compare
AO353_04325 +0.9 1.7 hypothetical protein compare
AO353_01810 +0.9 4.8 branched-chain amino acid ABC transporter substrate-binding protein compare
AO353_13865 +0.8 2.7 hypothetical protein compare
AO353_02550 +0.8 3.8 TetR family transcriptional regulator compare
AO353_06725 +0.8 1.5 hypothetical protein compare
AO353_26115 +0.8 1.8 hypothetical protein compare
AO353_19290 +0.8 2.2 molybdenum cofactor sulfurase compare
AO353_14380 +0.8 2.8 pantoate--beta-alanine ligase compare
AO353_09560 +0.8 1.1 type VI secretion protein compare
AO353_05415 +0.8 1.9 hypothetical protein compare
AO353_20810 +0.8 2.0 hypothetical protein compare
AO353_25575 +0.8 2.7 GNAT family acetyltransferase compare
AO353_18715 +0.8 1.7 histidine kinase compare
AO353_08620 +0.8 1.4 hypothetical protein compare
AO353_05090 +0.8 4.0 2-alkenal reductase compare
AO353_12085 +0.8 3.8 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
AO353_24120 +0.8 1.9 hypothetical protein compare
AO353_16355 +0.8 2.2 TetR family transcriptional regulator compare
AO353_22250 +0.7 2.4 NADPH-dependent FMN reductase compare
AO353_18770 +0.7 3.3 flagellar biosynthesis protein FlhA compare
AO353_27585 +0.7 2.1 DNA repair protein compare
AO353_08645 +0.7 2.0 transporter compare
AO353_18290 +0.7 3.5 FMN-dependent NADH-azoreductase compare
AO353_13840 +0.7 2.6 histidine kinase compare
AO353_18680 +0.7 3.0 flagellar M-ring protein FliF compare
AO353_17520 +0.7 1.9 hypothetical protein compare
AO353_01915 +0.7 1.3 GlcG protein compare
AO353_22520 +0.7 1.4 hypothetical protein compare
AO353_22930 +0.7 2.2 hypothetical protein compare
AO353_24760 +0.7 2.3 glutamine amidotransferase compare
AO353_03495 +0.7 2.5 glycine cleavage system protein H compare
AO353_13415 +0.7 3.0 TetR family transcriptional regulator compare
AO353_28320 +0.7 1.8 aminopeptidase compare
AO353_07465 +0.7 1.8 addiction module toxin RelE compare
AO353_09885 +0.7 1.4 transcriptional regulator PhoU compare
AO353_25585 +0.7 1.1 argininosuccinate lyase compare
AO353_01190 +0.7 1.4 hypothetical protein compare
AO353_10895 +0.7 3.6 hypothetical protein compare
AO353_25845 +0.7 1.9 GNAT family acetyltransferase compare
AO353_23725 +0.7 2.8 hypothetical protein compare
AO353_02775 +0.7 2.4 3-methyladenine DNA glycosylase compare
AO353_08565 +0.7 5.0 spermidine/putrescine ABC transporter permease compare
AO353_21410 +0.7 1.6 hypothetical protein compare
AO353_24685 +0.7 1.2 hypothetical protein compare
AO353_22280 +0.7 1.3 hypothetical protein compare
AO353_08100 +0.7 2.0 chemotaxis protein CheY compare
AO353_08875 +0.7 1.1 amino acid ABC transporter ATP-binding protein compare
AO353_02120 +0.7 5.3 hypothetical protein compare
AO353_12845 +0.7 4.2 peptide methionine sulfoxide reductase compare
AO353_26100 +0.7 1.3 lysozyme compare
AO353_05895 +0.6 2.5 RpiR family transcriptional regulator compare
AO353_19280 +0.6 1.6 hypothetical protein compare
AO353_12575 +0.6 2.0 type II secretion system protein GspJ compare
AO353_24595 +0.6 1.2 hypothetical protein compare
AO353_19755 +0.6 2.2 cytosine deaminase compare
AO353_02890 +0.6 3.8 pilus assembly protein PilZ compare
AO353_28080 +0.6 1.9 hypothetical protein compare
AO353_20205 +0.6 2.7 hypothetical protein compare
AO353_23005 +0.6 1.9 hypothetical protein compare
AO353_16635 +0.6 2.3 pyocin R2, holin compare
AO353_27420 +0.6 2.3 DNA-binding protein compare
AO353_02465 +0.6 1.1 hypothetical protein compare
AO353_01015 +0.6 1.5 hypothetical protein compare
AO353_09770 +0.6 2.7 hypothetical protein compare
AO353_18745 +0.6 1.0 flagellar biosynthesis protein flip compare
AO353_18965 +0.6 3.1 chemotaxis protein compare
AO353_08570 +0.6 4.2 transporter compare
AO353_09775 +0.6 1.8 hypothetical protein compare
AO353_04225 +0.6 2.7 chemotaxis protein CheA compare
AO353_18730 +0.6 2.2 flagellar motor switch protein FliM compare
AO353_17380 +0.6 2.4 hypothetical protein compare
AO353_02115 +0.6 4.7 TenA family transcriptional regulator compare
AO353_24830 +0.6 1.7 enoyl-CoA hydratase compare
AO353_27415 +0.6 1.4 hypothetical protein compare
AO353_24980 +0.6 2.4 formate dehydrogenase accessory protein FdhE compare
AO353_12940 +0.6 3.3 glycosyl transferase compare
AO353_23400 +0.6 1.3 hypothetical protein compare
AO353_29270 +0.6 1.7 type VI secretion system protein compare
AO353_04940 +0.6 3.4 hypothetical protein compare
AO353_21085 +0.6 3.0 lysine transporter LysE compare
AO353_12060 +0.6 1.6 acetyltransferase compare
AO353_11545 +0.6 2.9 sulfate ABC transporter permease compare
AO353_03970 +0.6 4.4 diguanylate cyclase compare
AO353_23930 +0.6 2.1 chemotaxis protein CheY compare
AO353_18685 +0.6 3.2 flagellar motor switch protein FliG compare
AO353_22830 +0.6 2.3 cupin compare
AO353_00025 +0.6 2.4 phenazine biosynthesis protein PhzF compare
AO353_07890 +0.6 3.4 alginate lyase compare
AO353_02130 +0.6 1.9 disulfide bond formation protein DsbA compare
AO353_13965 +0.6 2.2 large-conductance mechanosensitive channel compare
AO353_21770 +0.6 1.5 3-hydroxybutyrate dehydrogenase compare
AO353_18595 +0.6 2.1 flagellar biosynthesis protein FlgF compare
AO353_23540 +0.6 2.7 benzoate 1,2-dioxygenase small subunit compare
AO353_19840 +0.6 1.9 hypothetical protein compare
AO353_14495 +0.5 3.2 acetolactate synthase 3 catalytic subunit compare
AO353_23715 +0.5 2.9 hypothetical protein compare
AO353_18925 +0.5 0.5 ATPase compare
AO353_07610 +0.5 0.9 hypothetical protein compare
AO353_09450 +0.5 1.8 type VI secretion protein compare
AO353_01900 +0.5 3.0 hypothetical protein compare
AO353_02220 +0.5 0.6 hypothetical protein compare
AO353_20255 +0.5 1.7 transcriptional regulator compare
AO353_11965 +0.5 2.5 hypothetical protein compare
AO353_23895 +0.5 1.3 phospholipase compare
AO353_08575 +0.5 4.0 ABC transporter substrate-binding protein compare
AO353_11165 +0.5 3.4 hypothetical protein compare
AO353_08595 +0.5 3.1 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) compare
AO353_23620 +0.5 1.0 hypothetical protein compare
AO353_02990 +0.5 1.8 6,7-dimethyl-8-ribityllumazine synthase compare
AO353_26935 +0.5 1.2 LysR family transcriptional regulator compare
AO353_22485 +0.5 1.9 hypothetical protein compare
AO353_06710 +0.5 0.9 riboflavin synthase subunit alpha compare
AO353_07460 +0.5 1.2 DNA-binding protein compare
AO353_10200 +0.5 1.8 lysine transporter LysE compare
AO353_06745 +0.5 1.0 zinc-binding protein compare
AO353_21320 +0.5 1.5 glutathione S-transferase compare
AO353_19740 +0.5 1.7 MFS transporter compare
AO353_24605 +0.5 1.2 hypothetical protein compare
AO353_13290 +0.5 2.2 transglutaminase compare
AO353_03820 +0.5 1.6 Asp/Glu/hydantoin racemase compare
AO353_21540 +0.5 1.7 type VI secretion protein compare
AO353_08525 +0.5 1.9 purine nucleoside phosphorylase compare
AO353_09745 +0.5 1.0 hypothetical protein compare
AO353_00495 +0.5 1.1 DNA topoisomerase I compare
AO353_21620 +0.5 4.0 peptidylprolyl isomerase compare
AO353_00210 +0.5 2.5 terminase compare
AO353_27220 +0.5 1.8 hypothetical protein compare
AO353_02545 +0.5 3.2 MFS transporter compare
AO353_21675 +0.5 2.3 phospholipase compare
AO353_28300 +0.5 1.6 cupin compare
AO353_19705 +0.5 2.4 VWA containing CoxE family protein compare
AO353_19565 +0.5 1.7 vanillate O-demethylase oxidoreductase VanB compare
AO353_16025 +0.5 1.5 hypothetical protein compare
AO353_24940 +0.5 2.3 nitrogen fixation protein NifU compare
AO353_00850 +0.5 1.8 ribonuclease activity regulator protein RraA compare
AO353_00165 +0.5 2.4 lysis protein compare
AO353_10320 +0.5 1.0 hypothetical protein compare
AO353_04490 +0.5 2.9 murein transglycosylase compare
AO353_23830 +0.5 1.7 lactate dehydrogenase compare
AO353_07190 +0.5 3.4 S-adenosylmethionine decarboxylase proenzyme compare
AO353_18755 +0.5 2.3 flagellar biosynthetic protein FliR compare
AO353_09525 +0.5 2.5 ABC transporter ATP-binding protein compare
AO353_12760 +0.5 2.5 hypothetical protein compare
AO353_11565 +0.5 3.0 Crp/Fnr family transcriptional regulator compare
AO353_20585 +0.5 1.5 TetR family transcriptional regulator compare
AO353_20660 +0.5 1.3 pseudouridine synthase compare
AO353_28520 +0.5 2.1 LysR family transcriptional regulator compare
AO353_10890 +0.5 2.1 methionine ABC transporter ATP-binding protein compare
AO353_03830 +0.5 0.6 antibiotic biosynthesis monooxygenase compare
AO353_14570 +0.5 2.1 chromosome partitioning protein compare
AO353_27550 +0.5 0.8 hypothetical protein compare
AO353_12220 +0.5 2.4 hypothetical protein compare


Specific Phenotypes

For 9 genes in this experiment

For stress Cholin acetate in Pseudomonas fluorescens FW300-N2E3

For stress Cholin acetate across organisms