Experiment set4IT070 for Pseudomonas fluorescens FW300-N2E3

LB with Cholin acetate 38 mM

Group: stress
Media: LB + Cholin acetate (38 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 970 B3,B4

Specific Phenotypes

For 9 genes in this experiment

For stress Cholin acetate in Pseudomonas fluorescens FW300-N2E3

For stress Cholin acetate across organisms

SEED Subsystems

Subsystem	#Specific
Anaerobic respiratory reductases	1
Glycine and Serine Utilization	1
Glycine cleavage system	1
Photorespiration (oxidative C2 cycle)	1
Purine conversions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lysine biosynthesis
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Purine metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation III	1	1	1
glycine cleavage	3	3	1
L-ornithine biosynthesis II	3	3	1
glycine biosynthesis II	3	3	1
L-proline biosynthesis III (from L-ornithine)	3	3	1
L-arginine degradation I (arginase pathway)	3	2	1
L-arginine degradation VI (arginase 2 pathway)	4	3	1
L-arginine degradation XIII (reductive Stickland reaction)	5	5	1
L-ornithine biosynthesis I	5	5	1
L-Nδ-acetylornithine biosynthesis	7	5	1
L-citrulline biosynthesis	8	7	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-lysine biosynthesis I	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
superpathway of L-citrulline metabolism	12	9	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
aspartate superpathway	25	22	1