Experiment set4IT070 for Pseudomonas fluorescens FW300-N2E3

Compare to:

LB with Cholin acetate 38 mM

Group: stress
Media: LB + Cholin acetate (38 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 970 B3,B4

Specific Phenotypes

For 9 genes in this experiment

For stress Cholin acetate in Pseudomonas fluorescens FW300-N2E3

For stress Cholin acetate across organisms

SEED Subsystems

Subsystem #Specific
Anaerobic respiratory reductases 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Photorespiration (oxidative C2 cycle) 1
Purine conversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosine nucleotides degradation III 1 1 1
L-ornithine biosynthesis II 3 3 1
glycine biosynthesis II 3 3 1
glycine cleavage 3 3 1
L-proline biosynthesis III (from L-ornithine) 3 3 1
L-arginine degradation I (arginase pathway) 3 2 1
L-arginine degradation VI (arginase 2 pathway) 4 3 1
L-ornithine biosynthesis I 5 5 1
L-arginine degradation XIII (reductive Stickland reaction) 5 5 1
L-Nδ-acetylornithine biosynthesis 7 5 1
L-citrulline biosynthesis 8 7 1
L-arginine biosynthesis I (via L-ornithine) 9 9 1
L-lysine biosynthesis I 9 9 1
L-arginine biosynthesis III (via N-acetyl-L-citrulline) 9 8 1
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of L-citrulline metabolism 12 9 1
superpathway of arginine and polyamine biosynthesis 17 16 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 22 1