Experiment set4IT069 for Pseudomonas fluorescens FW300-N2E3

LB with Cholin acetate 19 mM

Group: stress
Media: LB + Cholin acetate (19 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 970 B1,B2

Specific Phenotypes

For 11 genes in this experiment

For stress Cholin acetate in Pseudomonas fluorescens FW300-N2E3

For stress Cholin acetate across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	2
Anaerobic respiratory reductases	1
Bacterial Chemotaxis	1
Flagellar motility	1
Glycine and Serine Utilization	1
Glycine cleavage system	1
Photorespiration (oxidative C2 cycle)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Glutamate metabolism
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
ammonia assimilation cycle III	3	3	1
glycine cleavage	3	3	1
glycine biosynthesis II	3	3	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
guanosine ribonucleotides de novo biosynthesis	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
L-glutamate and L-glutamine biosynthesis	7	6	1
superpathway of guanosine nucleotides de novo biosynthesis II	8	7	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of purine nucleotides de novo biosynthesis II	26	24	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	44	1