Experiment set4IT069 for Pseudomonas fluorescens FW300-N1B4

Compare to:

L-Glutamic acid monopotassium salt monohydrate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Glutamic acid monopotassium salt monohydrate (20 mM), pH=7
Culturing: pseudo1_N1B4_ML1, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 12/16/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Cplate1 C2

Specific Phenotypes

For 4 genes in this experiment

For carbon source L-Glutamic acid monopotassium salt monohydrate in Pseudomonas fluorescens FW300-N1B4

For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms

SEED Subsystems

Subsystem #Specific
Alginate metabolism 1
Cobalamin synthesis 1
Coenzyme B12 biosynthesis 1
De Novo Pyrimidine Synthesis 1
LMPTP YwlE cluster 1
Mannose Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyrimidine nucleobases salvage I 1 1 1
pyrimidine nucleobases salvage II 2 2 1
D-mannose degradation II 2 1 1
D-mannose degradation I 2 1 1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation 3 2 1
mannitol biosynthesis 3 2 1
GDP-mannose biosynthesis 4 4 1
superpathway of pyrimidine nucleobases salvage 4 4 1
mannitol degradation II 4 2 1
1,5-anhydrofructose degradation 5 2 1
β-(1,4)-mannan degradation 7 2 1
superpathway of pyrimidine ribonucleosides salvage 10 7 1
colanic acid building blocks biosynthesis 11 9 1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 13 1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis 14 7 1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 13 1
adenosylcobalamin biosynthesis II (aerobic) 33 31 1
adenosylcobalamin biosynthesis I (anaerobic) 36 29 1