Experiment set4IT069 for Acidovorax sp. GW101-3H11

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LB, pH10

Group: pH
Media: LB, pH=10
Culturing: acidovorax_3H11_ML3a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.3 generations
By: Mark on 6/10/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 33 genes in this experiment

SEED Subsystems

Subsystem #Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Acetyl-CoA fermentation to Butyrate 1
Bacterial hemoglobins 1
Butanol Biosynthesis 1
Coenzyme B12 biosynthesis 1
Folate Biosynthesis 1
Glutaredoxins 1
Heat shock dnaK gene cluster extended 1
Histidine Degradation 1
Isoleucine degradation 1
Lipoic acid metabolism 1
Methionine Biosynthesis 1
One-carbon metabolism by tetrahydropterines 1
Periplasmic disulfide interchange 1
Phenylpropanoid compound degradation 1
Phosphate metabolism 1
Polyhydroxybutyrate metabolism 1
Proline, 4-hydroxyproline uptake and utilization 1
Ribosome biogenesis bacterial 1
Serine-glyoxylate cycle 1
Ton and Tol transport systems 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
fatty acid β-oxidation III (unsaturated, odd number) 1 1 1
benzoyl-CoA biosynthesis 3 3 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 3
fatty acid β-oxidation I (generic) 7 5 4
acrylonitrile degradation I 2 1 1
oleate β-oxidation (thioesterase-dependent, yeast) 2 1 1
pseudouridine degradation 2 1 1
indole-3-acetate biosynthesis III (bacteria) 2 1 1
indole-3-acetate biosynthesis IV (bacteria) 2 1 1
oleate β-oxidation 35 29 16
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
glutaryl-CoA degradation 5 3 2
fatty acid β-oxidation II (plant peroxisome) 5 3 2
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 2 2
pyruvate fermentation to hexanol (engineered) 11 7 4
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 6 4
2-methyl-branched fatty acid β-oxidation 14 10 5
L-isoleucine degradation I 6 5 2
pyruvate fermentation to butanol II (engineered) 6 5 2
fatty acid salvage 6 5 2
propanoate fermentation to 2-methylbutanoate 6 5 2
valproate β-oxidation 9 7 3
chitin degradation II (Vibrio) 6 3 2
methyl ketone biosynthesis (engineered) 6 3 2
L-methionine salvage from L-homocysteine 3 1 1
L-arginine degradation X (arginine monooxygenase pathway) 3 1 1
oleate β-oxidation (reductase-dependent, yeast) 3 1 1
superpathway of acrylonitrile degradation 3 1 1
benzoyl-CoA degradation I (aerobic) 7 6 2
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 2
pyruvate fermentation to butanoate 7 3 2
L-methionine biosynthesis III 4 4 1
L-valine degradation I 8 6 2
pyruvate fermentation to butanol I 8 4 2
oleate β-oxidation (isomerase-dependent, yeast) 4 1 1
folate transformations III (E. coli) 9 7 2
phenylacetate degradation I (aerobic) 9 5 2
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 4 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 2
L-histidine degradation II 5 5 1
4-hydroxybenzoate biosynthesis III (plants) 5 4 1
L-glutamate degradation V (via hydroxyglutarate) 10 6 2
3-phenylpropanoate degradation 10 6 2
L-methionine biosynthesis I 5 3 1
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 3 1
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 2
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 1 1
folate transformations II (plants) 11 8 2
superpathway of phenylethylamine degradation 11 6 2
6-gingerol analog biosynthesis (engineered) 6 3 1
methylthiopropanoate degradation I (cleavage) 6 2 1
L-glutamate degradation VII (to butanoate) 12 3 2
folate transformations I 13 7 2
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 6 2
superpathway of glyoxylate cycle and fatty acid degradation 14 11 2
chitin degradation III (Serratia) 7 3 1
Spodoptera littoralis pheromone biosynthesis 22 4 3
L-tryptophan degradation III (eukaryotic) 15 7 2
superpathway of L-homoserine and L-methionine biosynthesis 8 6 1
glycerol degradation to butanol 16 10 2
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 4 2
superpathway of dimethylsulfoniopropanoate degradation 8 2 1
2-methylpropene degradation 8 2 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 2
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 5 2
superpathway of S-adenosyl-L-methionine biosynthesis 9 7 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 2
toluene degradation VI (anaerobic) 18 4 2
peptidoglycan recycling II 10 5 1
methyl tert-butyl ether degradation 10 2 1
gallate degradation III (anaerobic) 11 4 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
indole-3-acetate biosynthesis II 12 4 1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 1
androstenedione degradation I (aerobic) 25 6 2
platensimycin biosynthesis 26 7 2
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
1-butanol autotrophic biosynthesis (engineered) 27 19 2
androstenedione degradation II (anaerobic) 27 4 2
peptidoglycan recycling I 14 9 1
superpathway of testosterone and androsterone degradation 28 6 2
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 3
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 8 3
superpathway of L-methionine salvage and degradation 16 7 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 2 1
superpathway of cholesterol degradation III (oxidase) 49 4 2
aspartate superpathway 25 22 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 1