Experiment set4IT067 for Pseudomonas fluorescens FW300-N2E3
LB with Choline chloride 358 mM
Group: stressMedia: LB + Choline chloride (358 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 7/1/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 970 A5,A6
Specific Phenotypes
For 1 genes in this experiment
For stress Choline chloride in Pseudomonas fluorescens FW300-N2E3
For stress Choline chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| choline degradation I | 2 | 2 | 1 |
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| choline-O-sulfate degradation | 3 | 3 | 1 |