Experiment set4IT060 for Synechococcus elongatus PCC 7942

Bacitracin 0.0009765625 mg/ml

Group: stress
Media: BG11 + Bacitracin (0.000976563 mg/ml)
Culturing: SynE_ML6, 12 well microplate, Aerobic, at 30 (C), shaken=0 rpm
Growth: about 4.4 generations
By: Kelly on 6/20/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 19 genes in this experiment

For stress Bacitracin in Synechococcus elongatus PCC 7942

For stress Bacitracin across organisms

SEED Subsystems

Subsystem	#Specific
CO2 uptake, carboxysome	2
Bacterial Cell Division	1
Peptidoglycan Biosynthesis	1
Transcription initiation, bacterial sigma factors	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Oxidative phosphorylation
• Peptidoglycan biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1