Experiment set4IT060 for Pseudomonas fluorescens SBW25-INTG

Compare to:

no extra nitrogen source; with MOPS

Group: nitrogen source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 6 genes in this experiment

For nitrogen source D-Glucose in Pseudomonas fluorescens SBW25-INTG

For nitrogen source D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
Nitrate and nitrite ammonification 4
Bacterial Chemotaxis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
alkylnitronates degradation 2 2 1
nitrate reduction V (assimilatory) 2 1 1
oleate β-oxidation (isomerase-dependent, yeast) 4 2 1
fatty acid β-oxidation II (plant peroxisome) 5 4 1
propanoyl-CoA degradation II 5 4 1
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 4 1
fatty acid β-oxidation VII (yeast peroxisome) 5 3 1
(5Z)-dodecenoate biosynthesis II 6 6 1
methyl ketone biosynthesis (engineered) 6 4 1
6-gingerol analog biosynthesis (engineered) 6 3 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 4 2
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 4 2
jasmonic acid biosynthesis 19 7 3
fatty acid β-oxidation VI (mammalian peroxisome) 7 5 1
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 5 1
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 3 1
superpathway of glyoxylate cycle and fatty acid degradation 14 12 1
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 3 1
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) 14 2 1