Experiment set4IT056 for Bacteroides thetaiotaomicron VPI-5482
D-Glucose carbon source with 20 amino acid mix
Group: carbon sourceMedia: Varel_Bryant_medium + D-Glucose (20 mM) + 1X 20AA_mix
Culturing: Btheta_ML6, tube, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
20AA_mix 1x includes: 0.5 mM L-Arginine, 0.5 mM L-Histidine, 0.5 mM L-Lysine, 0.5 mM L-Aspartic Acid, 0.5 mM L-Glutamic acid monopotassium salt monohydrate, 0.5 mM L-Serine, 0.5 mM L-Threonine, 0.5 mM L-Asparagine, 0.5 mM L-Glutamine, 0.5 mM L-Cysteine hydrochloride monohydrate, 0.5 mM Glycine, 0.5 mM L-Proline, 0.5 mM L-Alanine, 0.5 mM L-Valine, 0.5 mM L-Isoleucine, 0.5 mM L-Leucine, 0.5 mM L-Methionine, 0.5 mM L-Phenylalanine, 0.5 mM L-tyrosine disodium salt, 0.5 mM L-Tryptophan
Specific Phenotypes
None in this experiment
For Bacteroides thetaiotaomicron VPI-5482 in carbon source experiments