Experiment set4IT054 for Pseudomonas fluorescens FW300-N2E2

N1B4wt with N2E2m denitrifying replicate C

Group: anaerobic
Media: UGA + Sodium pyruvate (15 mM) + Sodium nitrate (30 mM), pH=6.88
Culturing: pseudo6_N2E2_ML5, 100mL Serum Bottle, Anaerobic, at 30 (C), shaken=175 rpm
Growth: about 1.7 generations
By: Alex Aaring on 6/26/2015

Specific Phenotypes

For 5 genes in this experiment

For anaerobic Sodium pyruvate in Pseudomonas fluorescens FW300-N2E2

For anaerobic Sodium pyruvate across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	3
Ribonucleotide reduction	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Pyrimidine metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
nitrate reduction VII (denitrification)	5	5	1
nitrate reduction I (denitrification)	5	5	1