Experiment set4IT053 for Pseudomonas fluorescens FW300-N2E2

Compare to:

N1B4wt with N2E2m denitrifying replicate B

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_64 -5.9 -4.1 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_5006 -5.6 -13.4 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_2752 -4.8 -15.0 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_3661 -4.1 -7.9 hypothetical protein compare
Pf6N2E2_77 -4.1 -6.1 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3252 -4.0 -9.5 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3461 -3.9 -2.7 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_4597 -3.9 -10.8 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_71 -3.7 -6.6 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_4047 -3.6 -5.4 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4826 -3.5 -7.3 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3839 -3.5 -5.7 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3751 -3.4 -14.8 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_2574 -3.4 -2.3 Flagellar synthesis regulator FleN compare
Pf6N2E2_5177 -3.3 -12.2 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_5258 -3.3 -10.8 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_3841 -3.3 -7.7 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3610 -3.2 -5.1 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_2218 -3.2 -3.8 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_3752 -3.1 -19.3 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_3842 -3.1 -5.5 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_4473 -3.0 -10.3 HlyD family secretion protein compare
Pf6N2E2_4825 -3.0 -9.6 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_4048 -3.0 -9.2 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_5175 -3.0 -12.5 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3663 -2.9 -13.2 Glycosyl transferase compare
Pf6N2E2_4596 -2.9 -10.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_5720 -2.8 -14.9 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_735 -2.8 -15.2 Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2) compare
Pf6N2E2_66 -2.8 -9.5 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_2022 -2.7 -6.5 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_178 -2.7 -1.4 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_4564 -2.7 -10.0 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_5176 -2.7 -9.1 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3782 -2.7 -4.7 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3253 -2.7 -9.0 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_5337 -2.6 -4.9 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_3932 -2.6 -5.9 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_2074 -2.6 -2.6 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_5257 -2.6 -10.5 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_5160 -2.5 -2.2 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_737 -2.5 -2.3 Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4) compare
Pf6N2E2_2073 -2.4 -8.8 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_5338 -2.3 -7.5 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_3074 -2.3 -2.6 FIG00960973: hypothetical protein compare
Pf6N2E2_5291 -2.3 -17.2 Fumarylacetoacetase (EC 3.7.1.2) compare
Pf6N2E2_3264 -2.3 -1.6 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_2518 -2.3 -3.0 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_4206 -2.3 -2.1 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_4472 -2.2 -13.6 ABC-type multidrug transport system, permease component compare
Pf6N2E2_2217 -2.2 -6.3 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_736 -2.1 -3.2 hypothetical protein compare
Pf6N2E2_4372 -2.1 -5.9 FIG00460773: hypothetical protein compare
Pf6N2E2_3659 -2.1 -5.5 Protein fixF compare
Pf6N2E2_3645 -2.1 -8.9 Type I secretion outer membrane protein, TolC precursor compare
Pf6N2E2_2320 -2.1 -3.4 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_5339 -2.0 -6.4 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_2323 -2.0 -2.9 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_731 -2.0 -5.3 Respiratory nitrate reductase delta chain (EC 1.7.99.4) conserved
Pf6N2E2_5242 -1.9 -2.5 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_3170 -1.9 -7.3 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_2487 -1.9 -1.9 FIG00956090: hypothetical protein compare
Pf6N2E2_1490 -1.9 -3.0 hypothetical protein compare
Pf6N2E2_63 -1.9 -3.6 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_2509 -1.9 -2.2 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_2510 -1.9 -4.7 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_3258 -1.8 -6.7 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_3529 -1.8 -1.8 Toxin A compare
Pf6N2E2_5527 -1.8 -1.7 Cold shock protein CspC compare
Pf6N2E2_2465 -1.8 -7.3 Cys regulon transcriptional activator CysB compare
Pf6N2E2_5394 -1.8 -4.7 Molybdenum cofactor biosynthesis protein MoaE compare
Pf6N2E2_2318 -1.8 -7.5 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_2424 -1.8 -5.1 Molybdopterin-guanine dinucleotide biosynthesis protein MobA compare
Pf6N2E2_4471 -1.8 -7.5 ABC-type multidrug transport system, permease component compare
Pf6N2E2_3786 -1.7 -3.8 Twin-arginine translocation protein TatC compare
Pf6N2E2_3783 -1.7 -4.7 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_3664 -1.7 -8.1 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_2261 -1.7 -8.6 VacJ-like lipoprotein precursor compare
Pf6N2E2_3096 -1.6 -2.7 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_3260 -1.6 -5.2 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_5156 -1.6 -1.5 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_729 -1.6 -12.2 Respiratory nitrate reductase alpha chain (EC 1.7.99.4) conserved
Pf6N2E2_732 -1.6 -7.2 Respiratory nitrate reductase gamma chain (EC 1.7.99.4) conserved
Pf6N2E2_3630 -1.5 -5.1 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5679 -1.5 -4.5 hypothetical protein compare
Pf6N2E2_730 -1.5 -8.8 Respiratory nitrate reductase beta chain (EC 1.7.99.4) conserved
Pf6N2E2_3487 -1.5 -7.2 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_4584 -1.5 -2.9 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
Pf6N2E2_5704 -1.5 -6.1 Transcriptional regulatory protein PhoP compare
Pf6N2E2_5223 -1.5 -5.1 Molybdopterin biosynthesis protein MoeB compare
Pf6N2E2_2507 -1.4 -7.3 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_4351 -1.4 -7.1 Predicted signal transduction protein compare
Pf6N2E2_6140 -1.4 -1.0 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_2447 -1.4 -6.6 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_5902 -1.4 -3.3 hypothetical protein compare
Pf6N2E2_2927 -1.4 -3.7 Putative signal peptide protein compare
Pf6N2E2_4638 -1.4 -2.1 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_2508 -1.4 -4.1 hypothetical protein compare
Pf6N2E2_3658 -1.3 -6.2 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_3282 -1.3 -2.6 FIG005080: Possible exported protein compare
Pf6N2E2_4416 -1.3 -2.3 Tyrosine recombinase XerC compare
Pf6N2E2_5392 -1.3 -3.3 Molybdenum cofactor biosynthesis protein MoaC compare
Pf6N2E2_2176 -1.3 -3.1 Amidases related to nicotinamidase compare
Pf6N2E2_698 -1.3 -3.2 Heme d1 biosynthesis protein NirH compare
Pf6N2E2_4678 -1.3 -0.6 hypothetical protein compare
Pf6N2E2_4624 -1.3 -0.6 Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-) compare
Pf6N2E2_4059 -1.3 -1.4 DNA-binding protein HU-alpha compare
Pf6N2E2_2822 -1.2 -4.6 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_6100 -1.2 -2.2 tRNA-Val-TAC compare
Pf6N2E2_4640 -1.2 -0.8 hypothetical protein compare
Pf6N2E2_5328 -1.2 -4.0 FIG00958649: hypothetical protein compare
Pf6N2E2_5703 -1.2 -4.8 outer membrane protein H1 compare
Pf6N2E2_3835 -1.2 -3.9 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_3941 -1.2 -2.6 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_3942 -1.2 -5.7 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_4797 -1.2 -0.9 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_3460 -1.2 -1.1 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_4205 -1.2 -2.0 Chromosome (plasmid) partitioning protein ParA compare
Pf6N2E2_5647 -1.2 -3.9 Transcriptional regulator, TetR family compare
Pf6N2E2_2506 -1.2 -9.0 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_1463 -1.2 -3.1 Isochorismate pyruvate-lyase (EC 4.-.-.-) compare
Pf6N2E2_1296 -1.2 -4.9 DNA-binding response regulator, LuxR family compare
Pf6N2E2_2681 -1.1 -3.3 3-phosphoglycerate kinase compare
Pf6N2E2_3257 -1.1 -4.3 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_4418 -1.1 -0.9 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_145 -1.1 -6.2 putative exported protein compare
Pf6N2E2_2201 -1.1 -3.4 Transmembrane regulator protein PrtR compare
Pf6N2E2_3665 -1.1 -1.0 serine/threonine protein kinase compare
Pf6N2E2_144 -1.1 -9.5 Uncharacterized protein YtfM precursor compare
Pf6N2E2_431 -1.1 -5.1 Molybdenum transport system permease protein ModB (TC 3.A.1.8.1) compare
Pf6N2E2_2422 -1.1 -6.6 Molybdopterin biosynthesis protein MoeA compare
Pf6N2E2_701 -1.1 -4.6 Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL compare
Pf6N2E2_702 -1.0 -7.3 Heme d1 biosynthesis protein NirF compare
Pf6N2E2_1082 -1.0 -1.8 CopG protein compare
Pf6N2E2_4146 -1.0 -2.1 Membrane lipoprotein lipid attachment site containing protein USSDB6D compare
Pf6N2E2_3778 -1.0 -2.7 FIG00954300: hypothetical protein compare
Pf6N2E2_699 -1.0 -3.0 Heme d1 biosynthesis protein NirG compare
Pf6N2E2_4352 -1.0 -2.1 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_5133 -1.0 -2.7 FIG00953980: hypothetical protein compare
Pf6N2E2_432 -1.0 -5.4 Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1) compare
Pf6N2E2_5780 -1.0 -5.2 Periplasmic protease compare
Pf6N2E2_4733 -1.0 -1.6 hypothetical protein compare
Pf6N2E2_700 -1.0 -3.0 Heme d1 biosynthesis protein NirL compare
Pf6N2E2_1917 -1.0 -1.7 hypothetical protein compare
Pf6N2E2_37 -1.0 -2.1 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_3183 -1.0 -2.5 Believed to be involved in assembly of Fe-S clusters compare
Pf6N2E2_282 -1.0 -1.4 GNAT family acetyltransferase YjcF compare
Pf6N2E2_440 -1.0 -3.1 FIG00954920: hypothetical protein compare
Pf6N2E2_476 -0.9 -3.0 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_1116 -0.9 -2.3 Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) compare
Pf6N2E2_705 -0.9 -6.1 Cytochrome cd1 nitrite reductase (EC:1.7.2.1) compare
Pf6N2E2_3940 -0.9 -6.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_6065 -0.9 -2.5 ABC transporter in pyoverdin gene cluster, periplasmic component compare
Pf6N2E2_4052 -0.9 -1.6 FIG00955481: hypothetical protein compare
Pf6N2E2_697 -0.9 -6.1 Heme d1 biosynthesis protein NirJ compare
Pf6N2E2_2746 -0.9 -4.1 Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor compare
Pf6N2E2_4611 -0.9 -1.6 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_3039 -0.9 -3.6 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_2513 -0.9 -1.7 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_703 -0.9 -3.5 Cytochrome c55X precursor NirC compare
Pf6N2E2_2264 -0.9 -2.2 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf6N2E2_3667 -0.9 -0.8 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_4415 -0.9 -1.6 Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase compare
Pf6N2E2_5575 -0.9 -4.2 Glycerol uptake facilitator protein compare
Pf6N2E2_2753 -0.9 -1.9 HtrA protease/chaperone protein compare
Pf6N2E2_6099 -0.9 -1.2 tRNA-Glu-TTC compare
Pf6N2E2_3847 -0.9 -2.6 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_5130 -0.9 -0.9 hypothetical protein compare
Pf6N2E2_4422 -0.9 -2.2 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_1203 -0.9 -2.2 Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16) compare
Pf6N2E2_5677 -0.9 -1.5 Carbon storage regulator compare
Pf6N2E2_1099 -0.9 -1.9 Hypothetical protein GlcG in glycolate utilization operon compare
Pf6N2E2_4585 -0.8 -5.9 Cell division protein FtsX compare
Pf6N2E2_5574 -0.8 -5.6 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_2423 -0.8 -3.3 Molybdenum cofactor biosynthesis protein MoaB compare
Pf6N2E2_3834 -0.8 -4.9 Lipoprotein NlpD compare
Pf6N2E2_477 -0.8 -4.1 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_722 -0.8 -4.6 FIG139928: Putative protease compare
Pf6N2E2_6130 -0.8 -2.9 5S RNA compare
Pf6N2E2_572 -0.8 -2.9 hypothetical protein compare
Pf6N2E2_3617 -0.8 -3.5 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf6N2E2_5239 -0.8 -6.0 Putative sulfate permease compare
Pf6N2E2_4691 -0.8 -1.7 hypothetical protein compare
Pf6N2E2_302 -0.8 -3.6 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3908 -0.8 -2.4 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_3303 -0.8 -2.3 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf6N2E2_704 -0.8 -3.1 Cytochrome c551/c552 compare
Pf6N2E2_4751 -0.8 -1.9 response regulator compare
Pf6N2E2_5785 -0.8 -2.1 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5739 -0.8 -1.3 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family compare
Pf6N2E2_5188 -0.8 -2.6 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_750 -0.8 -2.5 Transcriptional regulator, DeoR family compare
Pf6N2E2_3132 -0.7 -2.3 FIG00954548: hypothetical protein compare
Pf6N2E2_34 -0.7 -2.8 Cytosine deaminase (EC 3.5.4.1) compare
Pf6N2E2_4824 -0.7 -3.2 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_5316 -0.7 -1.0 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_2755 -0.7 -1.4 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_5425 -0.7 -1.5 hypothetical protein compare
Pf6N2E2_2636 -0.7 -1.5 FIG027190: Putative transmembrane protein compare
Pf6N2E2_2072 -0.7 -1.5 hypothetical protein compare


Specific Phenotypes

For 6 genes in this experiment

For anaerobic Sodium pyruvate in Pseudomonas fluorescens FW300-N2E2

For anaerobic Sodium pyruvate across organisms