Experiment set4IT052 for Pseudomonas fluorescens SBW25-INTG

Temperature shift 10_to_30

Group: temperature shift
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM)
Culturing: PseudoSBW25_INTG_ML3, tube, Aerobic, at 10_to_30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 11 genes in this experiment

For temperature shift D-Glucose in Pseudomonas fluorescens SBW25-INTG

For temperature shift D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Biogenesis of cbb3-type cytochrome c oxidases	1
Folate Biosynthesis	1
Fructose utilization	1
Pentose phosphate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Biosynthesis of plant hormones
• Pentose phosphate pathway
• Fatty acid metabolism
• alpha-Linolenic acid metabolism
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
oleate β-oxidation (isomerase-dependent, yeast)	4	2	1
pentose phosphate pathway (non-oxidative branch) I	5	5	1
fatty acid β-oxidation II (plant peroxisome)	5	4	1
propanoyl-CoA degradation II	5	4	1
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent)	5	4	1
fatty acid β-oxidation VII (yeast peroxisome)	5	3	1
(5Z)-dodecenoate biosynthesis II	6	6	1
methyl ketone biosynthesis (engineered)	6	4	1
6-gingerol analog biosynthesis (engineered)	6	3	1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast)	12	4	2
10-cis-heptadecenoyl-CoA degradation (yeast)	12	4	2
jasmonic acid biosynthesis	19	7	3
fatty acid β-oxidation VI (mammalian peroxisome)	7	5	1
pentose phosphate pathway	8	8	1
formaldehyde assimilation II (assimilatory RuMP Cycle)	9	6	1
Rubisco shunt	10	8	1
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast)	10	5	1
formaldehyde assimilation III (dihydroxyacetone cycle)	12	10	1
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase)	13	3	1
superpathway of glyoxylate cycle and fatty acid degradation	14	12	1
docosahexaenoate biosynthesis III (6-desaturase, mammals)	14	3	1
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered)	14	2	1
Bifidobacterium shunt	15	12	1
superpathway of glucose and xylose degradation	17	16	1