Experiment set4IT051 for Pseudomonas fluorescens FW300-N2E3

LB with methylglyoxal 0.009 vol%

Group: stress
Media: LB + methylglyoxal (0.009 vol%)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 6/26/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 958 B1,B2

Specific Phenotypes

For 20 genes in this experiment

For stress methylglyoxal in Pseudomonas fluorescens FW300-N2E3

For stress methylglyoxal across organisms

SEED Subsystems

Subsystem	#Specific
Glutathione-dependent pathway of formaldehyde detoxification	2
Potassium homeostasis	2
Sodium Hydrogen Antiporter	2
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
DNA-replication	1
DNA repair, UvrABC system	1
DNA repair, bacterial	1
Glutathione-regulated potassium-efflux system and associated functions	1
Glutathione: Non-redox reactions	1
Methylglyoxal Metabolism	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1
Purine conversions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methane metabolism
• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Purine metabolism
• Glycine, serine and threonine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Retinol metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetaldehyde biosynthesis I	1	1	1
adenosine nucleotides degradation III	1	1	1
formaldehyde oxidation II (glutathione-dependent)	3	3	2
methylglyoxal degradation I	3	2	2
methylglyoxal degradation VIII	3	2	2
ethanol degradation I	2	2	1
superoxide radicals degradation	2	2	1
pyruvate fermentation to ethanol II	2	1	1
methanol oxidation to formaldehyde IV	2	1	1
ethanol degradation II	3	3	1
ethanol degradation IV	3	3	1
L-valine degradation II	3	2	1
pyruvate fermentation to ethanol III	3	2	1
pyruvate fermentation to ethanol I	3	2	1
L-leucine degradation III	3	2	1
L-isoleucine degradation II	3	2	1
L-methionine degradation III	3	1	1
reactive oxygen species degradation	4	4	1
queuosine biosynthesis I (de novo)	4	4	1
phytol degradation	4	3	1
superpathway of methylglyoxal degradation	8	4	2
L-phenylalanine degradation III	4	2	1
L-tyrosine degradation III	4	2	1
salidroside biosynthesis	4	1	1
ethanolamine utilization	5	5	1
acetylene degradation (anaerobic)	5	4	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
protein S-nitrosylation and denitrosylation	5	3	1
(S)-propane-1,2-diol degradation	5	2	1
phenylethanol biosynthesis	5	1	1
superpathway of C1 compounds oxidation to CO2	12	5	2
noradrenaline and adrenaline degradation	13	4	2
3-methylbutanol biosynthesis (engineered)	7	6	1
serotonin degradation	7	3	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	5	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	6	1
L-tryptophan degradation V (side chain pathway)	13	1	1
mixed acid fermentation	16	12	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	8	1
heterolactic fermentation	18	16	1
superpathway of anaerobic sucrose degradation	19	15	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	14	1
superpathway of N-acetylneuraminate degradation	22	15	1