Experiment set4IT051 for Pseudomonas fluorescens FW300-N1B4

LB with Fusidic acid sodium salt 0.125 mg/ml

Group: stress
Media: LB + Fusidic acid sodium salt (0.125 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1043 E1,E2

Specific Phenotypes

For 18 genes in this experiment

For stress Fusidic acid sodium salt in Pseudomonas fluorescens FW300-N1B4

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Cell Division	1
Bacterial Cytoskeleton	1
Biogenesis of cytochrome c oxidases	1
DNA repair, bacterial MutL-MutS system	1
Glycine and Serine Utilization	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Purine conversions	1
Pyruvate Alanine Serine Interconversions	1
Rhamnose containing glycans	1
Rubrerythrin	1
Serine-glyoxylate cycle	1
TCA Cycle	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Propanoate metabolism
• Biosynthesis of plant hormones
• Citrate cycle (TCA cycle)
• Fatty acid metabolism
• Purine metabolism
• Glycine, serine and threonine metabolism
• Cysteine metabolism
• Geraniol degradation
• Tyrosine metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• C5-Branched dibasic acid metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Limonene and pinene degradation
• Caprolactam degradation
• Alkaloid biosynthesis II
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
adenosine nucleotides degradation III	1	1	1
D-serine degradation	3	3	2
L-cysteine degradation II	3	3	2
L-tryptophan degradation II (via pyruvate)	3	2	2
glycine betaine degradation III	7	7	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	6	3
glycine degradation	3	3	1
L-methionine biosynthesis II	6	4	2
pyruvate fermentation to acetate V	3	2	1
pyruvate fermentation to acetate VI	3	1	1
L-mimosine degradation	8	4	2
dTDP-N-acetylviosamine biosynthesis	4	2	1
dTDP-6-deoxy-α-D-allose biosynthesis	4	2	1
dTDP-N-acetylthomosamine biosynthesis	4	2	1
dTDP-β-D-fucofuranose biosynthesis	4	2	1
glutathione-mediated detoxification I	8	3	2
dTDP-β-L-rhamnose biosynthesis	5	5	1
adipate degradation	5	5	1
octane oxidation	5	4	1
adipate biosynthesis	5	4	1
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis	5	2	1
dTDP-3-acetamido-α-D-fucose biosynthesis	5	2	1
dTDP-α-D-mycaminose biosynthesis	5	2	1
TCA cycle VIII (Chlamydia)	6	4	1
dTDP-sibirosamine biosynthesis	6	3	1
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-D-desosamine biosynthesis	6	2	1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis	6	2	1
incomplete reductive TCA cycle	7	3	1
dTDP-β-L-digitoxose biosynthesis	7	3	1
dTDP-β-L-olivose biosynthesis	7	3	1
dTDP-β-L-mycarose biosynthesis	7	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	12	2
purine nucleobases degradation II (anaerobic)	24	16	3
dTDP-β-L-megosamine biosynthesis	8	3	1
dTDP-β-L-4-epi-vancosamine biosynthesis	8	3	1
TCA cycle V (2-oxoglutarate synthase)	9	7	1
TCA cycle II (plants and fungi)	9	6	1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis	9	5	1
dTDP-α-D-forosamine biosynthesis	9	3	1
tunicamycin biosynthesis	9	2	1
TCA cycle I (prokaryotic)	10	8	1
TCA cycle III (animals)	10	6	1
anaerobic energy metabolism (invertebrates, mitochondrial)	10	4	1
superpathway of enterobacterial common antigen biosynthesis	10	3	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
reductive TCA cycle I	11	6	1
superpathway of glyoxylate bypass and TCA	12	10	1
reductive TCA cycle II	12	5	1
superpathway of anaerobic energy metabolism (invertebrates)	17	8	1
methylaspartate cycle	19	9	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of erythromycin biosynthesis	19	2	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	17	1
superpathway of megalomicin A biosynthesis	22	3	1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis	23	5	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	21	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	1