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Experiment set4IT051 for
Methanococcus maripaludis S2
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Formate McCas deepwell complete
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS01370
+2.7
21.8
acetate--CoA ligase family protein
compare
MMP
_RS01960
+2.2
1.8
hypothetical protein
compare
MMP
_RS06770
+1.2
9.4
DHH family phosphoesterase
compare
MMP
_RS07415
+0.9
6.3
molybdopterin-guanine dinucleotide biosynthesis protein MobB
compare
MMP
_RS07115
+0.9
7.4
MBL fold metallo-hydrolase
compare
MMP
_RS03215
+0.9
6.6
MBL fold metallo-hydrolase
compare
MMP
_RS05535
+0.8
4.7
hypothetical protein
compare
MMP
_RS01775
+0.8
2.1
TIGR00297 family protein
compare
MMP
_RS08660
+0.7
3.4
DHH family phosphoesterase
compare
MMP
_RS08275
+0.6
5.4
hypothetical protein
compare
MMP
_RS01420
+0.6
2.4
hypothetical protein
compare
MMP
_RS00675
+0.6
1.3
nitroreductase family protein
compare
MMP
_RS07345
+0.6
3.3
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS06480
+0.6
4.3
ABC transporter substrate-binding protein
compare
MMP
_RS01570
+0.6
1.8
hypothetical protein
compare
MMP
_RS08705
+0.5
4.2
sulfopyruvate decarboxylase subunit beta
compare
MMP
_RS02605
+0.5
1.0
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS03465
+0.5
1.4
adenine phosphoribosyltransferase
compare
MMP
_RS04145
+0.5
1.7
hypothetical protein
compare
MMP
_RS06545
+0.5
2.9
ferredoxin family protein
compare
MMP
_RS07890
+0.5
1.9
OB-fold nucleic acid binding domain-containing protein
compare
MMP
_RS08615
+0.5
1.6
flagellar protein G
compare
MMP
_RS01260
+0.5
2.4
HDIG domain-containing protein
compare
MMP
_RS07085
+0.5
3.1
ribonuclease HII
compare
MMP
_RS08775
+0.5
3.3
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS03385
+0.5
1.0
cation diffusion facilitator family transporter
compare
MMP
_RS01880
+0.5
1.6
DegT/DnrJ/EryC1/StrS family aminotransferase
compare
MMP
_RS06165
+0.4
3.0
ABC transporter permease
compare
MMP
_RS07130
+0.4
3.5
coenzyme F420 hydrogenase subunit gamma
compare
MMP
_RS08340
+0.4
1.8
4Fe-4S binding protein
compare
MMP
_RS07815
+0.4
1.1
DUF2120 domain-containing protein
compare
MMP
_RS07860
+0.4
0.6
hypothetical protein
compare
MMP
_RS07095
+0.4
1.3
PspC domain-containing protein
compare
MMP
_RS06550
+0.4
2.9
AMP-binding protein
compare
MMP
_RS07805
+0.4
2.4
ABC transporter permease
compare
MMP
_RS07850
+0.4
2.2
prephenate dehydratase
compare
MMP
_RS07820
+0.4
2.5
UPF0104 family protein
compare
MMP
_RS01885
+0.4
2.0
Gfo/Idh/MocA family oxidoreductase
compare
MMP
_RS07125
+0.4
2.4
coenzyme F420-reducing hydrogenase FrhD protein
compare
MMP
_RS01525
+0.4
1.3
nucleoside-diphosphate kinase
compare
MMP
_RS07120
+0.4
3.2
coenzyme F420 hydrogenase subunit alpha
compare
MMP
_RS08365
+0.4
2.4
energy conserving hydrogenase EhbF
compare
MMP
_RS06535
+0.4
2.2
2-oxoacid:acceptor oxidoreductase family protein
compare
MMP
_RS00945
+0.4
0.9
PadR family transcriptional regulator
compare
MMP
_RS00060
+0.4
0.8
3-dehydroquinate synthase II
compare
MMP
_RS01620
+0.4
1.9
hypothetical protein
compare
MMP
_RS06540
+0.4
1.6
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMP
_RS05975
+0.4
1.1
hypothetical protein
compare
MMP
_RS08310
+0.4
1.4
TMEM165/GDT1 family protein
compare
MMP
_RS03395
+0.4
2.1
hypothetical protein
compare
MMP
_RS06585
+0.3
1.2
DMT family protein
compare
MMP
_RS08315
+0.3
1.2
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMP
_RS07800
+0.3
2.4
ATP-binding cassette domain-containing protein
compare
MMP
_RS08595
+0.3
0.7
flagellar protein C
compare
MMP
_RS06290
+0.3
2.1
DNA repair and recombination protein RadA
compare
MMP
_RS06170
+0.3
2.5
ABC transporter ATP-binding protein
compare
MMP
_RS06900
+0.3
1.5
coenzyme F420-reducing hydrogenase FrhD protein
compare
MMP
_RS03800
+0.3
2.0
hypothetical protein
compare
MMP
_RS09050
+0.3
1.6
CAP domain-containing protein
compare
MMP
_RS00080
+0.3
1.7
RecB-family nuclease
compare
MMP
_RS07840
+0.3
1.7
ribonuclease III
compare
MMP
_RS08620
+0.3
2.0
flagellar accessory protein FlaH
compare
MMP
_RS06335
+0.3
1.5
HAD family hydrolase
compare
MMP
_RS00665
+0.3
1.8
hypothetical protein
compare
MMP
_RS04985
+0.3
2.0
DUF2180 family protein
compare
MMP
_RS08500
+0.3
2.0
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMP
_RS00155
+0.3
1.5
heavy metal-binding domain-containing protein
compare
MMP
_RS06555
+0.3
1.0
cupin domain-containing protein
compare
MMP
_RS08670
+0.3
1.8
metallophosphoesterase
compare
MMP
_RS06575
+0.3
1.2
fluoride efflux transporter CrcB
compare
MMP
_RS06570
+0.3
1.3
DUF190 domain-containing protein
compare
MMP
_RS01195
+0.3
0.7
metalloregulator ArsR/SmtB family transcription factor
compare
MMP
_RS04520
+0.3
1.8
nucleoside deaminase
compare
MMP
_RS03115
+0.3
2.5
hypothetical protein
compare
MMP
_RS08350
+0.3
0.6
hypothetical protein
compare
MMP
_RS07615
+0.3
2.0
energy-coupling factor ABC transporter permease
compare
MMP
_RS01110
+0.3
1.9
FmdE family protein
compare
MMP
_RS05835
+0.3
0.4
secondary thiamine-phosphate synthase enzyme YjbQ
compare
MMP
_RS07135
+0.3
2.1
coenzyme F420 hydrogenase subunit beta
compare
MMP
_RS08580
+0.3
2.0
flagellin
compare
MMP
_RS07705
+0.3
2.1
transcriptional regulator
compare
MMP
_RS05660
+0.3
1.8
phosphate ABC transporter substrate-binding protein
compare
MMP
_RS08980
+0.3
1.4
hypothetical protein
compare
MMP
_RS00655
+0.3
1.3
hypothetical protein
compare
MMP
_RS08280
+0.3
1.3
proteasome assembly chaperone family protein
compare
MMP
_RS04480
+0.3
2.2
lysine 2 3-aminomutase
compare
MMP
_RS05970
+0.2
1.2
class II SORL domain-containing protein
compare
MMP
_RS04305
+0.2
0.9
hypothetical protein
compare
MMP
_RS00400
+0.2
1.7
tributyrin esterase
compare
MMP
_RS00185
+0.2
2.2
potassium channel protein
compare
MMP
_RS01125
+0.2
1.8
thioredoxin family protein
compare
MMP
_RS07650
+0.2
2.0
TldD/PmbA family protein
compare
MMP
_RS08130
+0.2
2.0
IGHMBP2 family helicase
compare
MMP
_RS03300
+0.2
0.4
TOBE domain-containing protein
compare
MMP
_RS06300
+0.2
1.8
ABC transporter substrate-binding protein
compare
MMP
_RS09035
+0.2
1.1
hypothetical protein
compare
MMP
_RS07910
+0.2
1.4
NAD(P)H-hydrate dehydratase
compare
MMP
_RS05110
+0.2
1.7
16S ribosomal RNA methyltransferase A
compare
MMP
_RS02705
+0.2
1.6
formylmethanofuran dehydrogenase subunit B
compare
MMP
_RS08640
+0.2
2.1
TIM barrel protein
compare
MMP
_RS05980
+0.2
1.6
ferritin
compare
MMP
_RS07335
+0.2
0.9
tRNA (cytidine(56)-2'-O)-methyltransferase
compare
MMP
_RS01630
+0.2
1.5
N-glycosylase/DNA lyase
compare
MMP
_RS00705
+0.2
0.6
hypothetical protein
compare
MMP
_RS00670
+0.2
1.4
TIM barrel protein
compare
MMP
_RS09065
+0.2
1.9
hypothetical protein
compare
MMP
_RS08245
+0.2
1.7
DUF4013 domain-containing protein
compare
MMP
_RS08410
+0.2
1.2
DUF169 domain-containing protein
compare
MMP
_RS06190
+0.2
1.5
tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
compare
MMP
_RS03330
+0.2
1.8
rubrerythrin family protein
compare
MMP
_RS06250
+0.2
1.6
hypothetical protein
compare
MMP
_RS01875
+0.2
1.1
N-acetyltransferase
compare
MMP
_RS08475
+0.2
1.2
ABC transporter permease
compare
MMP
_RS04270
+0.2
1.3
coenzyme F420 hydrogenase subunit alpha
compare
MMP
_RS07560
+0.2
1.2
prefoldin subunit alpha
compare
MMP
_RS02815
+0.2
0.6
hypothetical protein
compare
MMP
_RS08235
+0.2
1.5
hypothetical protein
compare
MMP
_RS02475
+0.2
0.8
hypothetical protein
compare
MMP
_RS08345
+0.2
1.6
4Fe-4S binding protein
compare
MMP
_RS08395
+0.2
1.4
DsrE family protein
compare
MMP
_RS03245
+0.2
1.2
DNA repair and recombination protein RadB
compare
MMP
_RS08610
+0.2
1.5
flagellar protein F
compare
MMP
_RS03275
+0.2
1.3
TIGR00266 family protein
compare
MMP
_RS07855
+0.2
1.4
class I SAM-dependent methyltransferase
compare
MMP
_RS02290
+0.2
1.6
MBL fold metallo-hydrolase
compare
MMP
_RS08360
+0.2
1.1
hypothetical protein
compare
MMP
_RS02495
+0.2
0.9
hypothetical protein
compare
MMP
_RS05825
+0.2
1.0
peptidyl-prolyl cis-trans isomerase
compare
MMP
_RS08400
+0.2
1.2
MJ0307 family thioredoxin
compare
MMP
_RS00795
+0.2
1.4
formate dehydrogenase H subunit alpha selenocysteine-containing
compare
MMP
_RS05175
+0.2
1.4
hypothetical protein
compare
MMP
_RS06530
+0.2
1.0
bifunctional hexulose-6-phosphate synthase/ribonuclease regulator
compare
MMP
_RS02000
+0.2
1.0
radical SAM protein
compare
MMP
_RS05865
+0.2
0.8
hypothetical protein
compare
MMP
_RS07620
+0.2
1.2
energy-coupling factor ABC transporter substrate-binding protein
compare
MMP
_RS01155
+0.2
1.2
XRE family transcriptional regulator
compare
MMP
_RS06155
+0.2
0.8
DUF4186 domain-containing protein
compare
MMP
_RS08930
+0.2
0.6
hypothetical protein
compare
MMP
_RS07185
+0.2
1.3
pyridoxal phosphate-dependent aminotransferase
compare
MMP
_RS02795
+0.2
1.4
sulfate permease
compare
MMP
_RS06610
+0.2
1.0
DUF2334 domain-containing protein
compare
MMP
_RS01900
+0.2
1.2
hypothetical protein
compare
MMP
_RS03885
+0.2
1.4
tyrosine-type recombinase/integrase
compare
MMP
_RS01275
+0.2
1.3
class III signal peptide-containing protein
compare
MMP
_RS01680
+0.2
1.3
phenylacetate--CoA ligase
compare
MMP
_RS02770
+0.2
1.2
hypothetical protein
compare
MMP
_RS05665
+0.2
1.1
phosphate ABC transporter permease subunit PstC
compare
MMP
_RS00760
+0.2
1.3
branched-chain-amino-acid transaminase
compare
MMP
_RS00055
+0.2
1.2
DUF2254 domain-containing protein
compare
MMP
_RS01860
+0.2
1.2
hypothetical protein
compare
MMP
_RS07330
+0.2
1.3
membrane protein
compare
MMP
_RS08770
+0.2
1.1
hypothetical protein
compare
MMP
_RS01785
+0.2
1.3
hypothetical protein
compare
MMP
_RS01550
+0.2
1.2
DUF1624 domain-containing protein
compare
MMP
_RS00240
+0.2
1.5
type II secretion system F family protein
compare
MMP
_RS01515
+0.2
1.3
PINc/VapC family ATPase
compare
MMP
_RS06185
+0.2
1.2
thermonuclease family protein
compare
MMP
_RS01925
+0.2
0.8
DUF1616 domain-containing protein
compare
MMP
_RS07140
+0.2
1.3
TIGR00341 family protein
compare
MMP
_RS04965
+0.2
1.3
thioredoxin family protein
compare
MMP
_RS08470
+0.2
1.0
ATP-binding cassette domain-containing protein
compare
MMP
_RS08285
+0.2
1.1
serine/threonine protein kinase
compare
MMP
_RS08335
+0.2
0.9
NADH-quinone oxidoreductase subunit B family protein
compare
MMP
_RS00375
+0.2
1.2
ammonium transporter
compare
MMP
_RS03440
+0.2
0.3
MoaD/ThiS family protein
compare
MMP
_RS07590
+0.1
1.2
hypothetical protein
compare
MMP
_RS00105
+0.1
1.0
hypothetical protein
compare
MMP
_RS08480
+0.1
1.3
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS06060
+0.1
0.8
hypothetical protein
compare
MMP
_RS00285
+0.1
1.1
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS04030
+0.1
0.9
hypothetical protein
compare
MMP
_RS01470
+0.1
0.8
ATP-binding cassette domain-containing protein
compare
MMP
_RS07915
+0.1
1.1
DUF4870 domain-containing protein
compare
MMP
_RS03900
+0.1
1.1
minichromosome maintenance protein MCM
compare
MMP
_RS01430
+0.1
1.1
mechanosensitive ion channel family protein
compare
MMP
_RS08835
+0.1
1.0
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS08855
+0.1
1.1
hypothetical protein
compare
MMP
_RS02880
+0.1
1.2
AmmeMemoRadiSam system radical SAM enzyme
compare
MMP
_RS02895
+0.1
1.0
DHH family phosphoesterase
compare
MMP
_RS05895
+0.1
1.2
metallophosphoesterase
compare
MMP
_RS07150
+0.1
1.1
MTH895/ArsE family thioredoxin-like protein
compare
MMP
_RS01675
+0.1
0.5
hypothetical protein
compare
MMP
_RS00345
+0.1
1.0
DUF2666 domain-containing protein
compare
MMP
_RS08455
+0.1
0.8
multiprotein bridging factor aMBF1
compare
MMP
_RS07570
+0.1
1.1
nucleotidyltransferase family protein
compare
MMP
_RS07985
+0.1
1.1
nitroreductase family protein
compare
MMP
_RS03130
+0.1
1.0
hypothetical protein
compare
MMP
_RS09055
+0.1
0.2
CAP domain-containing protein
compare
MMP
_RS02325
+0.1
1.0
epoxyqueuosine reductase
compare
MMP
_RS01160
+0.1
1.0
DMT family transporter
compare
MMP
_RS05900
+0.1
1.1
tetratricopeptide repeat protein
compare
MMP
_RS03535
+0.1
0.6
helix-turn-helix transcriptional regulator
compare
MMP
_RS08485
+0.1
1.0
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS08405
+0.1
0.9
MFS transporter
compare
MMP
_RS00650
+0.1
0.9
ABC transporter ATP-binding protein
compare
MMP
_RS08090
+0.1
0.6
hypothetical protein
compare
MMP
_RS02590
+0.1
0.9
low molecular weight phosphatase family protein
compare
MMP
_RS00085
+0.1
1.0
DNA cytosine methyltransferase
compare
MMP
_RS02365
+0.1
0.9
nitrogen fixation
compare
MMP
_RS08665
+0.1
1.1
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t2 experiments