Experiment set4IT050 for Pseudomonas fluorescens FW300-N1B4
LB with Fusidic acid sodium salt 0.0625 mg/ml
Group: stressMedia: LB + Fusidic acid sodium salt (0.0625 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1043 D7,D8
Specific Phenotypes
For 8 genes in this experiment
For stress Fusidic acid sodium salt in Pseudomonas fluorescens FW300-N1B4
For stress Fusidic acid sodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycine and Serine Utilization | 1 |
| Peptidoglycan Biosynthesis | 1 |
| Purine Utilization | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| L-cysteine degradation II | 3 | 3 | 2 |
| D-serine degradation | 3 | 3 | 2 |
| L-tryptophan degradation II (via pyruvate) | 3 | 2 | 2 |
| glycine betaine degradation III | 7 | 7 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 6 | 3 |
| glycine degradation | 3 | 3 | 1 |
| L-methionine biosynthesis II | 6 | 4 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 12 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 3 |