Experiment set4IT050 for Sinorhizobium meliloti 1021

LB with Polymyxin B sulfate 0.00005 mg/ml

Group: stress
Media: LB + Polymyxin B sulfate (5e-05 mg/ml)
Culturing: Smeli_ML6, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.3 generations
By: Mark on 3/13/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: redo_stress2 C6

Specific Phenotypes

For 11 genes in this experiment

For stress Polymyxin B sulfate in Sinorhizobium meliloti 1021

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem	#Specific
dTDP-rhamnose synthesis	2
Ammonia assimilation	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glutamine synthetases	1
Peptidoglycan Biosynthesis	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1
Rhamnose containing glycans	1
SigmaB stress responce regulation	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism
• Starch and sucrose metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Nitrogen metabolism
• Biosynthesis of vancomycin group antibiotics

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine biosynthesis I	1	1	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	2	2
ammonia assimilation cycle I	2	2	1
ammonia assimilation cycle II	2	1	1
CDP-6-deoxy-D-gulose biosynthesis	5	3	2
ammonia assimilation cycle III	3	3	1
superpathway of ammonia assimilation (plants)	3	2	1
L-arginine degradation I (arginase pathway)	3	2	1
L-proline degradation I	3	2	1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis	9	5	3
L-aspartate degradation III (anaerobic)	3	1	1
L-aspartate degradation II (aerobic)	3	1	1
dTDP-N-acetylthomosamine biosynthesis	4	2	1
dTDP-N-acetylviosamine biosynthesis	4	2	1
dTDP-6-deoxy-α-D-allose biosynthesis	4	2	1
dTDP-β-D-fucofuranose biosynthesis	4	2	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	3	2
ethene biosynthesis II (microbes)	4	1	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis	5	2	1
dTDP-α-D-mycaminose biosynthesis	5	2	1
dTDP-3-acetamido-α-D-fucose biosynthesis	5	2	1
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-sibirosamine biosynthesis	6	3	1
dTDP-D-desosamine biosynthesis	6	2	1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis	6	2	1
L-glutamate and L-glutamine biosynthesis	7	5	1
dTDP-β-L-digitoxose biosynthesis	7	3	1
dTDP-β-L-olivose biosynthesis	7	3	1
dTDP-β-L-mycarose biosynthesis	7	2	1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis	23	5	3
dTDP-β-L-4-epi-vancosamine biosynthesis	8	3	1
dTDP-β-L-megosamine biosynthesis	8	3	1
dTDP-α-D-forosamine biosynthesis	9	3	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
superpathway of enterobacterial common antigen biosynthesis	10	2	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
streptomycin biosynthesis	18	3	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of erythromycin biosynthesis	19	2	1
superpathway of megalomicin A biosynthesis	22	3	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1